广东部分零售畜禽产品沙门菌生化型和血清型分析  

Biochemical and serological characterization Salmonella from retail meats in Guangdong Province

作　　者：杨小鹃[1,2,3,4] 吴清平[1,2,3,4] 张菊梅 董晓晖[1,2,3,4] 刘盛荣[1,2,3,4] 

机构地区：[1]广东省微生物研究所 [2]广东省华南应用微生物重点实验室-省部共建国家重点实验室培育基地 [3]广东省菌种保藏与应用重点实验室 [4]广东省微生物应用新技术公共实验室,广州510070

出　　处：《卫生研究》2013年第4期615-618,共4页Journal of Hygiene Research

基　　金：科技部港澳台科技合作专项(No.2013DFH30070);广州市科技计划项目(No.2010U1-E00591;2010U1-E00611)

摘　　要：目的研究分析畜禽产品中沙门菌的生化型和血清型,了解沙门菌在畜禽产品中的污染状况,为确保食品安全提供依据。方法依据GB 4789.4—2010《食品安全国家标准食品微生物学检验沙门氏菌检验》,对样品进行检测,利用API 20E试剂条对菌株进行鉴定分析,采用玻片凝集法测定沙门菌的血清型。结果畜禽产品209份,检出沙门菌阳性样品42份,总检出率为20.10%,污染样品多来自于生鸭肉(检出率为69.23%),生鸡肉(37.14%)、生牛肉(20.00%)、生猪肉(16.67%)。API鉴定出46株沙门菌,分为7种不同生化谱的沙门菌API生化型,主要的2种为6704752和6704552。畜禽肉中沙门菌主要血清型为德尔卑沙门菌(21.74%)、鼠伤寒沙门菌(10.87%)、肠炎沙门菌(8.70%)、茨昂威沙门菌(8.70%)、印地安纳沙门菌(8.70%)、韦太夫雷登沙门菌(8.70%)。结论广东畜禽产品沙门菌污染情况较为严重,沙门菌菌株生化型和血清型表现为多样性的特征。Objective Salmonella isolates from meat in Guangdong Province were characterized to determine their phenotype and serotype,to investigate contamination status of meats by Salmonella and provide scientific basis for detection and further control.Methods 209 meat samples were detected according to methods GB 4789.4—2010 National food safety standard Food microbiological examination： Salmonella.The API 20E system was used to identify isolates.Serotyping of Salmonella isolates was performed by slide agglutination method using antisera to Salmonella.Results According to the detection results of 209 meat samples,forty-six Salmonella strains were isolated from 42 meat samples,the positive rate was 20.10%.The majority of positive samples were fresh meat,69.23% in duck,37.14% in chicken,20.00% in beef and 16.67% in pock.The strains identified as Salmonella by the API 20E were distributed in 7 profile numbers with two of them being predominant（6704752 and 6704552）.The most common serotypes were Derby（21.74%）,Typhimurium（10.87%）,Enteritidis（8.70%）,Tshiongwe（8.70%）,Indiana（8.70%）,Weltevreden（8.70%）.Conclusion Salmonella present in retail meats were common and phenotypically diverse in Guangdong Province.

关 键 词：畜禽产品 沙门菌 鉴定 API20E 血清型 

分 类 号：R155.51[医药卫生—营养与食品卫生学] TS207.4[医药卫生—公共卫生与预防医学]