脑出血后星形胶质细胞表达运铁蛋白LCN2  

LCN2 expression in astrocytes after ICH in rats

作　　者：于澎[1] 颜秀丽[2] 黄朔[2] 吴江[2] 奚国华[3] 华雅[3] 董铭[2] 

机构地区：[1]吉林大学白求恩第二医院眼科,吉林长春130041 [2]吉林大学白求恩第一医院神经内科,吉林长春130021 [3]美国密歇根大学神经外科

出　　处：《中风与神经疾病杂志》2013年第7期598-601,共4页Journal of Apoplexy and Nervous Diseases

摘　　要：目的探讨脑出血时脑内LCN2表达情况。方法雄性SD大鼠基底节区注射自体动脉血(100μl)。对照组大鼠术式相同注射等量生理盐水。Western blot(以β-actin比值矫正)和免疫组化方法分析脑LCN2表达。结果 LCN2阳性细胞只在脑出血同侧基底节区表达(对侧不表达),对照组出血同侧LCN2表达极微弱。Western blotting定量发现在出血侧基底节区,出血后1、3、7d LCN2蛋白水平显著升高,14d后下降。脑出血3d时出血侧LCN2蛋白水平较对侧高71倍(2.70±0.46 vs 0.04±0.01,P<0.001),较对照组高84倍(0.92±0.14 vs0.01±0.01,P<0.001)。LCN2阳性细胞形态似为星形胶质细胞,免疫组化双染证实LCN2与胶质细胞标志物GFAP共定位。结论本研究提示脑出血后释放的铁离子诱导载铁蛋白LCN2表达。LCN2在血肿清除铁转运过程中可能起重要作用。Objective To investigate the role of iron in brain LCN2 expression after ICH.Methods Male SpragueDawley rats had an intracaudate injection of autologous blood（100μl）.Control rats received needle insertion or saline injection.Brain LCN2 expression was determined by Western blot analysis（and expressed as a ratio to β-actin） and immunohistochemistry.Results LCN2 positive cells were found in the ipsilateral but not the contralateral basal ganglia after ICH.Western blotting showed that LCN2 protein levels in the ipsilateral basal ganglia were significantly increased at day 1,remained high at days 3 and 7,and then decreased markedly at day 14 after ICH.Immunoreactivity of LCN2 was very weak in sham brain but increased significantly after ICH.LCN2 positive cells were astrocyte-like.By Western blot,the LCN2/βactin ratio the ipsilateral basal ganglia was 71-fold higher than in contralateral basal ganglia（2.70 ± 0.46 vs 0.04 ± 0.01,P 0.001） and 84-fold higher than in sham animals（0.92 ± 0.14 vs 0.01 ± 0.01,P 0.001） at 3 days after ICH.Double-labeling showed that LCN2 immunoreactivity co-localized with glial fibrillary acid protein immunoreactivity after ICH.LCN2 immunoreactivity rarely co-localized with neuronal nuclear antigen in cortex.Conclusion Our findings indicate a role of iron in brain LCN2 upregulation after ICH.It also suggests that LCN2 may play an important role in the handling of iron released from the hematoma during clot resolution.

关 键 词：脑出血 LCN2 铁转运 

分 类 号：R743.34[医药卫生—神经病学与精神病学]