改良型固定化Pseudomonas oleovorans DT4降解四氢呋喃的研究  

Biodegradation of Tetrahydrofuran by Combined Immobilized of Pseudomonas oleovorans DT4

作　　者：邵钱[1] 叶杰旭[1] 欧阳杜娟[1] 陈建孟[1] 陈东之[1] 

机构地区：[1]浙江工业大学生物与环境工程学院,杭州310014

出　　处：《环境科学》2013年第8期3251-3256,共6页Environmental Science

基　　金：国家自然科学项目(20907043);国家高技术研究发展计划(863)项目(2012AA063102);浙江省科技厅创新团队项目(20110150)

摘　　要：成功构建出了新型的海藻酸钠-活性炭纤维复合固定化Pseudomonas oleovorans DT4来去除四氢呋喃,并优化了该复合固定化载体组分的含量,发现在海藻酸钠含量为3%,活性炭纤维含量为1.5%条件下,制备成细胞浓度为6×109g-1的协同固定化细胞在初始THF浓度为360 mg·L-1时的降解速率达到最大为24.0 mg·(L.h)-1,同时其机械强度也得到了显著的提升.此外与游离菌体相比,该固定化细胞在不同温度和pH条件下的降解速率表现得更加稳定,且去除效果均能稳定在80%以上.在改进的反应体系中,协同固定化小球高效降解THF的重复利用次数达到80批以上,显示出该载体良好的可行性.A new composite matrix,calcium alginate（CA） coupled with activated carbon fiber（ACF） was designed to immobilize the cells of Pseudomonas oleovorans DT4 for tetrahydrofuran（THF） degradation.The average removal rate of the CA-ACF immobilized cells reached 24.0 mg·（L·h）-1 with an initial THF concentration of 360 mg·L-1 when the concentration of CA and ACF was 3% and 1.5% respectively.The mechanical strength of the mobilized cells was also significantly improved with the addition of ACF.Compared to the free suspended cells,higher stable removal efficiency（more than 80%） of CA-ACF cells was detected under different conditions of temperature and pH.The feasibility of the newly designed matrix was also reflected by the repeated batch degradation which showed that the removal activity decreased insignificantly after 80 cycles with the modified reaction system（PNS）.

关 键 词：四氢呋喃 固定化 海藻酸钠 活性炭纤维 生物降解 

分 类 号：X172[环境科学与工程—环境科学]