两种实验设计研究DES和EV对MCF-7细胞增殖的联合作用  

Study of Combined Effects of DES and EV on the Proliferation of MCF-7 Cells by Two Experimental Designs

作　　者：刘倩[1] 雷炳莉[1] 安静[1] 尚羽[1] 钟玉芳[1] 康佳[1] 文育[1] 

机构地区：[1]上海大学环境与化学工程学院,环境污染与健康研究所,上海200444

出　　处：《环境科学》2013年第8期3303-3308,共6页Environmental Science

基　　金：上海市重点学科项目(S30109);上海市优秀青年基金项目(shu10040)

摘　　要：研究了己烯雌酚(DES)和戊酸雌二醇(EV)对MCF-7细胞增殖的单一毒性和等浓度配比下的联合毒性作用,用相加指数法进行了联合毒性作用类型的判断,同时进行了联合毒性作用的3×3析因实验设计研究结果作为对比.结果表明,EV的24、48和72 h对MCF-7细胞增殖率的EC50值分别为6.02、0.40和0.33 nmol·L-1,DES的24、48和72 h的EC50值分别为5.90、6.98和2.90 nmol·L-1,等浓度配比下其联合毒性的EC50值分别为2.33、0.71和0.39 nmol·L-1,用相加指数判断其联合作用类型,在24 h显示为协同作用,其它两个时间段显示为拮抗效应,但从数值上可以发现协同和拮抗效应均不强,接近相加的联合效应类型.析因实验结果显示24、48和72 h这3个染毒时间段均显示了相加作用类型.析因实验与等浓度配比下获得的联合毒作用类型基本一致,但析因实验设计更为简捷方便,同时避免了由于计算EC50值产生的偏差对联合效应类型的影响.The single toxicity of diethylstilbestrol（DES） and β-estradiol 17-valerate（EV） and the joint toxicity of their binary mixtures in equiconcentration to the proliferation of MCF-7 cells were investigated,respectively.Additive index（AI） method was adopted to evaluate the joint toxicity effect.At the same time,3×3 factorial experimental design was used to verify the joint toxicity types derived from equiconcentration of DES and EV.The results show that the EC50 values of single EV and DES for 24,48 and 72 h are 6.02,0.40 and 0.33 nmol·L-1 and 5.90,6.98 and 2.90 nmol·L-1,respectively.The EC50 values of the binary mixtures of DES and EV for 24,48 and 72 h are 2.33,0.71 and 0.39 nmol·L-1.The binary joint effects of DES and EV for 24 h were synergistic,and the joint effects of DES and EV for 48 and 72 h were antagonistic.But synergistic and antagonistic effects are not strong;their values can be found close to the values of additive effects.Factorial experiment results show that combined effects of DES and EV to proliferation of MCF-7 cells for 24,48 and 72 h three exposure periods are additive effect types.The consistent joint combined effect types can be drawn from both factorial experimental design and equiconcentration ratio of DES and EV to the proliferation of MCF-7 cells.However,the factorial experimental design is simpler and more convenient,and can avoid unnecessary mistakes due to the derivation of EC50 values.

关 键 词：联合毒性 己烯雌酚 戊酸雌二醇 等浓度配比 析因实验设计 

分 类 号：X13[环境科学与工程—环境科学] X171.5