大鼠开胸手术后慢性疼痛模型的构建  

Establishment of chronic post-surgical pain evoked by thoracotomy in a rat in vivo model

作　　者：王海棠[1] 刘薇[1] 黄宇光[1] 

机构地区：[1]北京协和医院麻醉科,100730

出　　处：《临床麻醉学杂志》2013年第7期700-702,共3页Journal of Clinical Anesthesiology

摘　　要：目的探讨建立大鼠开胸术后慢性疼痛模型的稳定性和可重复性。方法雄性SD大鼠36只,采用随机数字表法将大鼠随机均分为三组:空白组、假手术组和模型组。参考Buvanendran法建立大鼠开胸术后慢性疼痛模型。观察并记录术前、术后1、7、14、21、32及40d的机械缩足阈值(MWT)和冷刺激反应。结果术后1、7、21、32和40d模型组大鼠MWT显著低于术前、空白组和假手术组(P<0.01);术后1、7、21、32和40d模型组冷刺激反应明显高于术前、空白组和假手术组(P<0.01);模型组开胸术后慢性疼痛的发生率为41.7%。结论进一步证实了大鼠开胸术后慢性疼痛模型的稳定性和可重复性,从而为探索和验证手术后慢性疼痛的发生机制及预防和治疗措施提供了一个新的平台。Objective To establish a chronic postoperative pain evoked by thoracotomy in a rat in vivo model. Methods Thirty-six male SD rats were randomly divided into three groups（n= 12） ： control group, sham group and model group. Animal model of postthoraeotomy pain was constructed as Buvanendran described. Results Compared with the sham group, the threshold to mechanical stimulation started to decrease at 1st postoperative day（P〈0. 01）and increased gradually after 3 days. However, mechanical withdrawal threshold （MWT） of some of these rats declined significantly on 7th postoperative day and continued to decrease until 32nd postoperative day. Therefore, the incidence rate of chronic postthoracotomy pain was 41.7% in model group. In addition, the numbers of scratches following the application of acetone markedly increased in model group rats compared with those in the sham group（P〈0.01）. There were no significant differences between the model group and control group in mechanical allodynia and cold hypersensitibity. Conclusion The results of the present study suggest that the model of persistent postoperative pain evoked by thoracotomy is stable and repeatable,which may help to study the mechanisms and treatment of chronic postoperative pain.

关 键 词：开胸术后慢性疼痛 动物模型 疼痛行为学 

分 类 号：R-332[医药卫生]