孔石莼多糖及其磺化衍生物HU体外结合脂类和胆固醇的研究  

Research on binding ablities to lipid and cholesterol of Ulvan and sulfonation derivative HU in vitro

作　　者：王凯[1] 李敏[1] 张言捷[1] 綦慧敏[1,2] 

机构地区：[1]潍坊医学院,山东潍坊261053 [2]天然药物活性物质与功能国家重点实验室,中国医学科学院北京协和医学院药物研究所,北京100050

出　　处：《药学研究》2013年第7期379-383,共5页Journal of Pharmaceutical Research

基　　金：国家自然科学基金(41206129/D0609);山东省自然科学基金(ZR2012DL13);潍坊医学院大学生创新计划(KX2012040)

摘　　要：目的研究孔石莼多糖及其磺化孔石莼多糖HU在体外结合脂类和胆固醇的能力,并进一步阐明其可能作用的机制。方法在体外模拟人体消化环境,测定孔石莼多糖U及磺化孔石莼多糖HU对脂肪、胆固醇和胆酸盐的结合能力。结果孔石莼多糖进行磺化后显著增强了它对胆固醇和胆盐的结合能力,但相对减弱了它对脂肪的结合能力。结论孔石莼多糖及其磺化衍生物在体外对脂类和胆固醇具有很强的结合能力,孔石莼多糖中硫酸基的含量是影响其吸收脂类和胆固醇的重要因素。Objective To investigate the combining abilities to lipid and cholesterol of Ulvan and sulfonated derivative HU in vitro,and further to clarify the possible mechanism. Methods In vitro simulation digestive environment,the combining abilities of Ulvan （ U ） and HU were determined including to fat, cholesterol and bile salts, and furthermore, the mechanisms of U and HU were discussed in vitro combining with the lipids and cholesterol. Results HU showed higher combining abilities to cholesterol and bile salt,but showed weaker binding ability to fat. Conclusion U and HU showed strong binding abilities to lipids and cholesterol, and the sulfate content was the important factor on absorbing lipids and cholesterol.

关 键 词：孔石莼 多糖 磺化衍生物 胆盐 胆固醇 结合能力 

分 类 号：R285.5[医药卫生—中药学]