菲律宾蛤仔酶解多肽抗前列腺癌DU-145细胞的活性研究  

Study on Antiproliferative Activity of Ruditapes philippinarum Hydrolysate on Human Prostate Cancer DU-145 Cell

作　　者：李连军[1] 徐律[1] 杨最素[1] 丁国芳[1] 孙瑜[1] 

机构地区：[1]浙江海洋学院食品与医药学院,浙江省海洋生物医用制品重点工程技术研究中心,浙江舟山316004

出　　处：《安徽农业科学》2013年第15期6717-6718,6762,共3页Journal of Anhui Agricultural Sciences

基　　金：浙江省自然科学基金(LY12C20008)

摘　　要：[目的]探讨菲律宾蛤仔酶解多肽抗人前列腺癌DU-145细胞增殖的活性。[方法]对菲律宾蛤仔内脏进行酶解,经超滤截取3 kD以下的多肽,应用MTT法、倒置显微镜观察和AO/EB染色等方法检测其体外抗DU-145细胞的活性。[结果]MTT法检测结果表明不同浓度的多肽对DU-145细胞有明显的生长抑制作用。通过倒置显微镜观察和AO/EB荧光双染观察到DU-145细胞出现细胞凋亡的形态学特征。[结论]菲律宾蛤仔3 kD以下的多肽具有抑制DU-145细胞生长,呈浓度和时间依赖性,并诱导细胞凋亡作用。[Objective] To study Philippines clam enzymolysis peptide on prostate cancer DU-145 cells proliferation activity in vitro.[Method] Digestive enzyme was used to enzymolysis the Philippine clam guts,3 kD molecular weight cut-off membranes were used to fractionate the Ruditapes philippinarum hydrolysate.The anti-proliferation activity and the early apoptosis of Ruditapes philippinarum peptides in vitro were detected by MTT,inverted microscope and AO/EB double staining respectively.[Result] Determined by MTT method,different concentration of peptides to DU-145 cells had obvious growth inhibition.Cell apoptosis morphological feature was observed under AO/EB double staining and inverted microscope.[Conclusion] Ruditapes philippinarum under 3 kD enzyme solution of molecular weight with inhibition of DU-145 cells growth,induce prostate cancer cells apoptosis,and a concentration and time dependence.

关 键 词：菲律宾蛤仔 酶解多肽 前列腺癌细胞 凋亡 

分 类 号：S944.47[农业科学—水产养殖]