仿生法评定饲料干物质消化率的影响因素  

Factors Affecting the Measure of the in vitro Dry Matter Digestibility of Feeds Using Simulative Digestion System

作　　者：陈亮[1] 张宏福[1] 高理想[1] 赵峰[1] 

机构地区：[1]中国农业科学院北京畜牧兽医研究所/动物营养学国家重点实验室,北京100193

出　　处：《中国农业科学》2013年第15期3199-3205,共7页Scientia Agricultura Sinica

基　　金：国家科技支撑计划项目课题(2012BAD39B01);国家重点实验室自主研究课题(2004DA125184G1104);公益性行业(农业)科研专项(200903006)

摘　　要：【目的】为确立饲料养分生物学效价仿生法的评定操作规程,探讨仿生法测定饲料干物质消化率的影响因素。【方法】试验采用单因素完全随机设计,共设6个处理,每个处理5个重复,每个重复使用1根仿生消化管,其中处理1—2、3—4、5—6分别考察透析袋的前处理方式、消化残渣脱脂和蠕动泵泵入方式对饲料体外干物质消化率的影响。【结果】①透析袋使用两种前处理方式,玉米、豆粕和麦麸干物质消化率无显著影响(P>0.05);②脱脂显著提高玉米、豆粕和麦麸的干物质和粗脂肪的消化率,干物质消化率提高约3%,粗脂肪消化率达到90%以上(P<0.05),但脱脂未对饲料其它养分的消化率产生影响(P>0.05);③蠕动泵泵入缓冲液方式对饲料干物质消化率有显著影响,注入式所测得的玉米、豆粕和麦麸的饲料干物质消化率显著低于吸入式(P<0.05),但是其测试的平行性较好。【结论】仿生法测试饲料体外干物质消化率时,消化残渣应进行脱脂,透析袋采用两种前处理方式均可,蠕动泵以注入方式更符合测试精度的要求。【Objective】 The objective of the present study was to determine the effects of the factors on the in vitro dry matter digestibility（DMD） of feeds using Simulative Digestion System.It would provide a method for meaning the DMD of feeds using Simulative Digestion System.【Method】 A single factor completely randomized design with 6 treatments was adopted and each treatment contained 5 replicates with 1 digestion tube per replicate.The treatments 1-2,3-4 and 5-6 were used to determine the effects of a preliminary method of dialysis tube,removing of the oil and fat from undigested residues,and running direction of peristaltic pump on the DMD of corn,soybean meal and wheat bran,respectively.【Result】 No significant differences were revealed on the DMD of feeds using two preliminary methods of dialysis tube（P〈0.05）.Removing of the oil and fat from undigested residues,the DMD of corn,soybean meal and wheat bran were increased by 3%,and the in vitro ether extract digestibility of feeds was more than 90%（P〈0.05）,but the in vitro digestibility of other nutrients was not affected by this method（P〈0.05）.The DMD of feeds was different in the pushing and pulling method of running direction of peristaltic pump（P〈0.05）.Although the DMD of feeds was less in the pushing method than in pulling,the results with well accuracy and precision were obtained in pushing method.【Conclusion】The removing of the oil and fat from undigested residues and the pushing direction of peristaltic pump are suitable for the determination of DMD of feeds using Simulative Digestion System.The preliminary of dialysis tube in two methods did not significantly affect the DMD of feeds.

关 键 词：仿生消化系统 体外干物质消化率 透析袋前处理 脱脂 缓冲液泵入方式 

分 类 号：S816[农业科学—饲料科学]