122例丙肝抗体阳性血清核酸及抗体复检结果分析  

Repeated detection results of nucleic acid and antibody in 122 serum samples positive with anti-HCV

作　　者：杨燕[1] 李洪波[2] 单万水[1] 韩红星[1] 李小勇[1] 

机构地区：[1]广东省深圳市第三人民医院检验科,518000 [2]广东医学院2008级实习生,510000

出　　处：《检验医学与临床》2013年第A01期39-41,共3页Laboratory Medicine and Clinic

摘　　要：目的对122例酶联免疫检测为丙型肝炎病毒抗体（HCV—Ab）阳性的样本血清，用重组免疫印迹法（RIBA）进行确证同时做荧光定量聚合酶链反应（PCR），确证检测的相关性。方法收集该院门诊及住院患者酶联免疫法检测丙型肝炎病毒抗体（HCV-Ab）呈反应性的样本血清122例，分别用重组免疫印迹确认试剂盒（RIBA）检测及荧光定量聚合酶链反应（PCR）进行检测。结果122例酶联免疫法检测HCV-Ab有反应性的样品中，用重组免疫印迹法（RIBA）做丙肝确认试验结果有阳性119例（S／CO值2．3～25．67，均值16．78，符合率97．5％）；不确定1例（S／CO为3．56占0．8％）；阴性2例（C／CO值小于5，均值为3．24，占1．7％）。荧光定量聚合酶链反应（PCR）检出阳性79例（检出率64．8％）。结论丙肝病毒抗体酶免法试剂可信度较高，但在S／CO值小于5的情况下应做重组免疫印迹确认试验来确证，减少假阳性的结果，不同标志物之间存在一定的联系。Objective To verify detection results of hepatitis C virus （HCV） by using recombinant immunob- lot assay（RIBA） and fluorescence quantitative PCR（FQ PCR） ,and to analyze relativity. Methods 122 serum samples positive with anti-HCV, detected by using enzyme linked immunosorbent assay （ELISA）, were detected for anti-HCV by ysing RIBA and for HCV RNA by using FQ^PCR. Results Among 122 serum samples, 119 cases were positive by RIBA detection （S/CO 2.3-25.67, averagel6.78, coincidence rate 97.5 %）, 1 cases was indeterminate （S/CO 3.56, rate 0.8%）, 2 cases were negative （S/CO〈5, average 3.24, rate 1.7%）. 79 cases were positive for FQ-PCR detection （64.8 %）. Conclusion The confidence of anti-HCV detection by ELISA could be high, but samples with S/CO〈5 should be detected by using RIBA. There might be some relations among the three makers of HCV.

关 键 词：丙型肝炎病毒抗体 丙肝确认试验 丙型肝炎病毒核酸 检测 

分 类 号：R737.9[医药卫生—肿瘤]