苦豆子内生细菌的分离、筛选及活性鉴定  

Isolation, screening and activity identification of endophytic bacteria in Sophora alopecuroides L.

作　　者：顾沛雯[1] 胡美娟[1] 周星辰[1] 

机构地区：[1]宁夏大学农学院,宁夏银川750021

出　　处：《中国植保导刊》2013年第7期9-12,8,共5页China Plant Protection

基　　金：宁夏自然科学基金(nz1002);国家自然科学基金(30960228)

摘　　要：对苦豆子健康植株和种子内的内生细菌进行分离,共获得41株内生细菌。同一植株分离内生细菌的数量:种子和根部最多,茎和叶部较少。采用平板对峙培养方法筛选出3株对植物病原菌有明显体外颉颃活性的菌株XKGKDB19、NDZKDB29和NDZKDB25。其中NDZKDB29菌株表现广谱抗菌活性。经16S rRNA基因序列分析鉴定,3株高活性菌株分属于黄芪叶瘤杆菌(Phyllobacterium ifriqiyense)、弯曲假单胞菌(Pseudomonas geniculata)和固氮阴沟肠细菌(Enterobacter cloacae)。41 strains of endophytic bacteria were isolated from health plant and seed of Sophora alopecuroides L.. Quantity of endophytic bacteria from different parts of S. alopecuroides varied and that in seeds and roots was more than those in stems and leaves of single plant. 3 strains, XKGKDB19, NDZKDB29 and NDZKDB25 with antagonistic activity against pathogen were selected by plate opposition culture method, and NDZKDB29 had broad-specificity antagonistic activity, and those 3 strains were identified as PhyUobacterium ifriqiyense, Pseudomonas geniculata and Enterobacter cloacae respectively by 16S rRNA gene sequences analysis.

关 键 词：苦豆子 内生细菌 抑菌活性 16S rRNA序列分析 

分 类 号：S476[农业科学—农业昆虫与害虫防治]