核因子-κB与肿瘤坏死因子α、白介素1β在口腔扁平苔藓中的表达及意义  

Expression of nuclear factor-kappa B,tumor necrosis factor-alpha and interleukin1β in oral lichen planus and the significance

作　　者：满昭昭[1] 许野[1] 朱兰兰[1] 王秀梅[1] 

机构地区：[1]哈尔滨医科大学附属第二医院口腔内科,哈尔滨150086

出　　处：《口腔医学》2013年第7期461-464,共4页Stomatology

基　　金：黑龙江省博士后科研启动基金(LRB05-279);黑龙江省自然科学基金(D201175)

摘　　要：目的检测核因子-kappaB(nuclear factor-kappa B,NF-κB)和肿瘤坏死因子α(tumor necrosis factor-alpha,TNF-α)、白介素1β(Interleukin1β,IL-1β)在口腔扁平苔癣组织中的表达并探讨3种因子在口腔扁平苔癣发病中的作用。方法采用SP免疫组化方法检测35例口腔扁平苔癣及10例正常口腔黏膜组织中NF-κB及TNF-α、IL-1β的表达情况,并进一步分析三者的相关性及各自与口腔扁平苔癣临床特征的关系。结果 NF-κB阳性染色主要定位于细胞的胞核和胞质,主要表达于棘层和基底层的角化细胞以及固有层淋巴细胞浸润带中。而TNF-α和IL-1β阳性染色定位于细胞质中,主要表达于口腔扁平苔癣中棘层和基底层角化细胞及固有层淋巴细胞浸润带中。三种因子的表达与临床分型有关,但与病损部位、性别、年龄关系不密切。结论口腔扁平苔癣中NF-κB和TNF-α、IL-1β高表达,且NF-κB与两者互为正相关关系,提示在OLP发病中TNF-α及IL-1β的高表达与NF-κB通路的活化密切相关。Objective To examine the expression of nuclear factor-kappa B（NF-κB）,tumor necrosis factor-alpha（TNF-α） and interleukin1β（IL-1β） in oral lichen planus（OLP） and to investigate the roles of these three cytokines in the development of OLP.Methods Immunohistochemical technique with SP method was used to detect the expression of NF-κB、TNF-α and IL-1β in 35 OLP cases and 10 normal oral mucosa specimens.Furthermore,their correlation and their respective relationship with clinical features of OLP were analyzed.Results NFκB positive staining was mainly found in cytoplasm and nucleu of cells,and it mainly expressed in spinous and basal epithelial keratinocytes and in lamina propria of filtrated lymphocytes.TNF-α positive and IL-1β positive stainings were mainly detected in cytoplasm and they mainly expressed in the spinous and basal keratinocytes and in filtrated lymphocytes.Expression of NF-κB and TNF-α、IL-1β might be related to clinical classification,but it had not close relationship with lesion sites,genders and ages.Conclusions High expression of NF-κB and TNF-α,IL-1β and the positive correlation of NF-κB and the other two in OLP suggest that NF-κB signal transduction loop activation has close relationship with overexpression of TNF-α and IL-1β in the pathogenesy of OLP.

关 键 词：扁平苔藓 口腔 核因子-ΚB 肿瘤坏死因子-α 白介素-1Β 

分 类 号：R781.59[医药卫生—口腔医学]