^60Coγ射线诱发小鼠外周血网织红细胞微核率的研究  

Micronucleated reticulocytes in the peripheral blood of mice exposed to ^60Coγ-rays

作　　者：朴春南[1] 刘建香[1] 高刚[1] 陈雪[1] 阮健磊[1] 苏旭[1] 

机构地区：[1]中国疾病预防控制中心辐射防护与核安全医学所辐射防护与核应急中国疾病预防控制中心重点实验室,北京100088

出　　处：《中华放射医学与防护杂志》2013年第3期242-244,共3页Chinese Journal of Radiological Medicine and Protection

摘　　要：目的通过观察不同剂量^60Coγ 射线照射后小鼠外周血网织红细胞微核率的变化，为使外周血网织红细胞微核成为探索快速高通量的生物剂量计提供科学依据。方法^60Coγ射线照射ICR小鼠，按不同照射剂量分为0、0．5、1、24、和8Gy组，眼球取血，流式细胞术（FCM）检测和显微镜观察小鼠外周血网织红细胞微核率的变化。结果流式细胞术检测网织红细胞微核率随剂量增加逐渐增加，2Gy达峰值，之后随剂量的增加而减少；显微镜观察的网织红细胞微核率变化趋势与流式细胞术检测结果基本一致。照射剂量在0～2Gy剂量范围内，小鼠的外周血网织红细胞微核率的剂量．效应关系满足直线模型（R^2 =0．9063），并且2Gy照射组与0Gy组相比差异有统计学意义（t=-2．856，P〈0．05）。结论流式细胞术检测外周血网织红细胞微核率，在一定剂量范围内可成为早期快速、高通量的辐射损伤生物剂量计。Objective To detect the changes of the percentage of micronucleated reticulocytes （MN-RET） in the peripheral blood of mice exposed to ^60Coγ-rays, in order to provide evidence for a new biomarker of radiation biodosimetry. Methods ICR mice were irradiated in whole body with 0, 0.5, 1 , 2, 4 and 8 Gy at a dose rate of 0.24 Gy/min. Peripheral blood was collected for MN-RET assay using a flow cytometry. Results The percentage of peripheral MN-RET increased steadily with irradiation doses up to 2 Gy and then had a downtrend beyond 2 Gy. The changes of MN-RET observed with a microscope were consistent with the results from flow cytometry. The dose response of the MN-RET fitted to a lineal model （R2 = 0. 9063） , and the MN-RET at 2 Gy was significantly higher than that of nonirradiated control （t = -2. 856,P 〈 0. 05 ）. Conclusion Percentage of peripheral MN-RET could be an early, rapid and high-throughput radiation bio-dosimeter in certain range of doses.

关 键 词：^60Coγ射线 生物剂量计 网织红细胞微核 流式细胞术 

分 类 号：R[医药卫生]