人IFN-λ1重组腺病毒对胃癌细胞增殖的影响及其机制研究  

Effects of hIFN-λ1 recombinant adenovirus on proliferation of gastric adenocarcinoma cell line and its mechanism

作　　者：刘峻[1,3] 严玉兰[2] 张金[2] 刘洋[2] 张杰[3] 梁冰[3] 步雪峰[1] 

机构地区：[1]江苏镇江市江苏大学附属人民医院普外科,212002 [2]江苏大学附属人民医院呼吸科 [3]江苏大学临床医学院

出　　处：《中华微生物学和免疫学杂志》2013年第7期507-512,共6页Chinese Journal of Microbiology and Immunology

基　　金：江苏省镇江市2011社发科技支撑项目（SH2011037）

摘　　要：目的探讨Ad-hIFN-λ1重组腺病毒对胃腺癌细胞SGC-7901增殖的影响及其机制。方法分别用Ad-hIFN-λ1重组腺病毒、Ad-LacZ空质粒及PBS对照组作用于人胃腺癌细胞SGC-7901，MTT法测定细胞增殖情况，RT-PCR检测胃癌SGC-7901中IFN-λ1mRNA的表达，免疫荧光法检测hIFN-λ1蛋白的表达，Tunel、流式细胞术检测细胞凋亡。结果Ad-hIFN-λ1转染胃腺癌细胞SGC-7901后，肿瘤细胞生长明显抑制，细胞中hIFN-λ1 mRNA、蛋白高效表达，细胞凋亡率明显高于Ad-LacZ组、PBS空白对照组。结论Ad-hIFN-λ1重组腺病毒转染至胃腺癌细胞SGC-7901后表达IFN-λ1，Ad-hIFN-λ1能够显著抑制人胃腺癌SGC-7901细胞生长，并可能通过诱导其凋亡而起作用。Objective To explore the effects of Ad-hIFN-λ1 recombinant adenovirus on prolifera- tion of gastric adenocareinoma cell line SGC-7901 and its mechanism. Methods Ad-hlFN-λ1 recombinant adenovirus and empty plasmid Ad-LacZ were respectively transfated to human gastric adenocarcinoma SGC- 7901 cells. The proliferation of transfected cells was detected by MTT assay. IFN-λ1l gene expression at mR- NA and protein levels in the ceils were measured by RT-PCR analysis and immunofluoreseenee assay, re- spectively. Tunel assay and flow eytometry were used to analyze the rate of cell apoptosis. Results The proliferation of gastric adenoeareinoma SGC-7901 cells were significantly inhibited with Ad-hlFN-λ1 inter- vention in accordance with highly expressed IFN-λ1at mRNA and protein levels, respectively. The apoptosis rate of Ad-hlFN-λ1 transfeeted cells was markedly higher than that of the empty plasmid Ad-LaeZ group and PBS blank control group. Conclusion The expression of hlFN-λ1 could be detected after transfection of Ad-hlFN-λ1 recombinant adenovirus into gastric adenocareinoma SGC-7901 cells. Ad-hlFN-λ1 could signifi- cantly inhibit the proliferation of gastric adenocarcinoma SGC-7901 cells by promoting the apoptosis of cancer cells.

关 键 词：胃癌 IFN-λ 重组病毒 凋亡 

分 类 号：R735[医药卫生—肿瘤]