增强大鼠神经干细胞生物活性的黄芪注射液  

Astragalus injection strengthens biological viability of rat neural stem cells

作　　者：张力[1] 罗秀成[1] 杨石照 张军峰[1] 杨吉平[1] 赵朝华[1] 张辉[2] 

机构地区：[1]西安医学院解剖教研室,陕西省西安市710021 [2]河北北方学院,河北省张家口市075000

出　　处：《中国组织工程研究》2013年第27期5057-5062,共6页Chinese Journal of Tissue Engineering Research

摘　　要：背景: 黄芪对神经功能缺损疾病治疗及神经再生的作用已受到神经科学和脑科学研究者的密切关注,其对神经干细胞的影响也成为一个新的探索方向。目的: 探索黄芪注射液对大鼠神经干细胞生物活性的影响。方法: 分离、培养Wistar大鼠胚胎神经干细胞。采用荧光免疫细胞化学法鉴定巢蛋白染色阳性,原代培养细胞传至第2代纯化后,随机分为对照组、50,200,400g/L黄芪注射液组分别培养6,12,24h后。采用MTT法检测细胞活性,通过比较细胞活性,选50g/L黄芪注射液组诱导分化7d后用免疫组化法检测神经元特异性烯醇化酶和胶质纤维酸性蛋白的表达。结果与结论: MTT显示药物作用6h,50,200,400g/L黄芪注射液组细胞的活性与对照组相比明显升高(P<0.05);但24h后不同质量浓度的黄芪注射液对细胞活性逐渐趋于一致(P>0.05)。免疫组织化学检测显示,与对照组相比,50g/L的黄芪注射液组诱导的细胞分化快速,细胞中神经元特异性烯醇化酶阳性细胞数量也明显增加(P<0.05)。实验提示黄芪注射液可以促进神经干细胞增殖,对细胞分化也具有一定促进作用。METHODS： Neural stem cells of Wistar rats were separated and cultured, immunofluorescence staining was applied to identify the neural stem cells. The purified cells were gained by the second subcultivation in vitro, and then the cells were randomly divided into control group and astragalus injection groups with various concentrations （50, 200, 400 g/L） to culture for 6, 12 and 24 hours. The activity of cells was tested by 3-（4,5-dimethyithiazol-2-yl）-2, 5-diphenyltetrazolium bromide assay, and then the immunohistochemistry was applied to detect the expressions of neuron-specific enolase and glial fibrillary acidic protein in the 50 g/L astragalus injection group after induced for 7 days. RESULTS AND CONCLUSION： The viability of neural stem cells increased significantly after intervention with different concentrations of astragalus injection for 6 hours as compared with the control group （P 〈 0.05）. However, there was no difference in the cell viability after treated with different concentrations of astragalus injection for 24 hours （P 〉 0.05）. Compared with the control group, the cells in the 50 g/L astragalus group differentiated rapidly, and the number of positive cells for neuron-specific enolase was increased significantly （P 〈 0.05）. The neural stem cells proliferation was hastened, and its differentiation was promoted by the interference of astragalus injection.

关 键 词：干细胞 干细胞与中医药 干细胞基础实验 中医药 黄芪注射液 神经干细胞 MTT 生物活性 细胞分化 干细胞图片文章 

分 类 号：R394.2[医药卫生—医学遗传学]