MMP-12基因多态性与中国北方人非小细胞肺癌易感性的关系  

Association between the matrix metalloproteinase - 12 gene polymorphism and susceptibility of non - small cell lung cancer in northern China

作　　者：王伟[1] 曾辉[2] 王瑞[2] 

机构地区：[1]河北省成安县人民医院外科,河北邯郸056700 [2]河北医科大学第四医院

出　　处：《西北国防医学杂志》2013年第4期301-303,共3页Medical Journal of National Defending Forces in Northwest China

基　　金：河北省卫生厅医学科学研究重点课题(20100123);河北省普通高等学校强势特色学科肿瘤学建设基金资助项目[冀教告(2005)52号]

摘　　要：目的:探讨MMP-12启动子区-82bp A/G单核苷酸多态性(SNP)与我国北方人非小细胞肺癌(NSCLC)遗传易感性的关系。方法:采用基于医院的病例-对照研究方法,收集300例非小细胞肺癌患者(其中鳞癌161例,腺癌139例)和300例健康对照个体的静脉抗凝血5 ml,以蛋白酶K消化-饱和氯化钠盐析法提取外周血白细胞DNA,采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法对基质金属蛋白酶MMP-12-82A/G单核苷酸多态性进行基因分型,比较非小细胞肺癌病例组与健康对照组之间等位基因及基因型分布。结果:非小细胞肺癌病例组中吸烟个体的比例(62.0%)明显高于健康对照组(36.3%),P<0.01,经年龄、性别校正后的OR值为3.74(95%CI=2.451～5.705)。健康对照组中的MMP-12基因启动子区转录起始点上游82 bp处A/G SNP的基因型分布符合Hardy-Weinberg平衡(P>0.05)。在病例组和健康对照组之间及根据吸烟状况和病理类型分层分析MMP-12基因-82A/G SNP基因型和等位基因频率分布均无显著性差异(P>0.05)。结论:吸烟可以显著增加非小细胞肺癌的发病风险。MMP-12基因-82A/G SNP与非小细胞肺癌的发病风险无关。Objective：To investigate the association between matrix metalloproteinases-12（MMP-12-82A/G SNP） polymorphism and susceptibiliy of non-small cell lung cancer（NSCLC） in northern China.Methods： This hospital-based case-control study included 300 patients with non-small cell lung cancer（161 squamous carcinoma and 139 adenocarcinoma） and 300 healthy controls.Total DNA was extracted form peripheral blood leucocyte using proteinase K digestion followed by a salting out procedure.Polymorphisms of MMP-12 gene were analyzed by PCR-restriction fragment length polymorphism（RFLP）.Results： The percentage of smokers in the patients（62.0%） was significantly higher than that in healthy controls（36.3%,P0.01）.Smoking may increase the risk of NSCLC（age and gender adjusted OR=3.74,95% CI=2.451-5.705）.The distribution of MMP-12-82 A/G SNP in the promoter region of healthy controls was not significantly different from the patients with NSCLC evaluated by Hardy-Weinberg equilibrium（P0.05）.The allelotype and genotype distribution of the MMP-12-82A/G SNP was not significantly different between NSCLC patients and healthy controls according to smoking status and histological type（P0.05）.Conclusion： Smoking increases the risk of developing NSCLC.The allelotype and genotype distribution of MMP-12-82A/G SNP in the NSCLC patients is not significantly different from that in healthy controls.

关 键 词：非小细胞肺癌 基质金属蛋白酶 多态性 单核苷酸 易感性 肿瘤 

分 类 号：R734.2[医药卫生—肿瘤]