家蝇胚胎细胞抗菌蛋白对肿瘤细胞A375抑制作用的研究  

The inhibitory action of antibacterial protein from the embryonic cells of the Musca domestica on melanoma cells A375

作　　者：张金花[1,2] 贺莉芳[1] 杨霞霞[1] 万启惠[1] 

机构地区：[1]遵义医学院寄生虫学教研室,贵州遵义563003 [2]川北医学院附属医院检验科,四川南充637000

出　　处：《川北医学院学报》2013年第4期322-324,共3页Journal of North Sichuan Medical College

基　　金：四川省卫生厅项目(100134)

摘　　要：目的:研究家蝇胚胎抗菌蛋白对肿瘤细胞A375生长的抑制作用。方法:利用脂多糖诱导家蝇胚胎细胞提取抗菌蛋白,将提取的抗菌蛋白用PBS配制成40、80、160、320及640μg/mL 5个浓度组,做黑色素瘤细胞A375的MTT实验,实验组采用等量PBS,观察家蝇胚胎细胞抗菌蛋白对A375的抑制作用,以人脐静脉血管内皮细胞作为正常对照组。结果:MTT检测显示五种浓度抗菌蛋白对A375均有抑制作用,与阴性组相比P<0.05,差异有显著性。各浓度组对人脐静脉血管内皮细胞无抑制作用,与阴性组相比P>0.05,差异无显著性。结论:家蝇胚胎细胞抗菌蛋白对肿瘤细胞A375具有明显抑制作用,而对正常人体细胞无抑制。Objective：To research the inhibitory action of the antibacterial protein from the embryonic cells of the Musea domestica for melanoma cells A375. Methods： The logarithmic growth phase＇ s embryonic cells of the Musca domestica were induced by LPS. The antibacterial protein was extracted and was prepared 40, 80, 160, 320 and 640 μg/mL five density groups and the human umbili- cal vein vascular endothelial cells were normal control group. The inhibitory action on melanoma cells A375 and the human umbilical vein vascular endothelial eells were made by MTT experiments. Results： The MTT examination demonstrated that the five density anti- bacterial proteins had all inhibition activities on melanoma cells A375, compared with the negative group, P 〈 0.05, and had no inhi- bition activities on human umbilical vein vascular endothelial cell, compared with the negative control group, P 〉 0.05. Conclusion： The antibacterial protein from the embryonic cell of the Musca domestiea has significant inhibitory activity on melanoma cells A375, and has no inhibition activity on normal human cells.

关 键 词：家蝇胚胎细胞 抗菌肽 抗菌蛋白 黑色素瘤细胞A375 人脐静脉血管内皮细胞 抑瘤活性 

分 类 号：R730.5[医药卫生—肿瘤]