白细胞介素4基因内含子3多态性与贲门癌和非贲门胃癌的易感性关系  

Correlation of interleukin-4 intron 3 polymorphisms with genetic susceptibilities to gastric cardiac carcinoma and nongastric cardiac carcinoma

作　　者：沈志勇[1] 林秀华[2] 彭孝纬[3] 程惠华[1] 

机构地区：[1]南京军区福州总医院放疗科,福州350025 [2]福建医科大学教学医院福建省福州肺科医院肿瘤科,350008 [3]福建医科大学省立临床医学院胃肠病研究所,350108

出　　处：《临床肿瘤学杂志》2013年第7期614-618,共5页Chinese Clinical Oncology

摘　　要：目的探讨白细胞介素4(IL-4)基因内含子3多态性与中国福建省贲门癌、非贲门胃癌遗传易感性的关系。方法采用序列特异性引物聚合酶链式反应(PCR-SSP)法和Sanger测序法检测中国福建省100例贲门癌、130例非贲门胃癌和113例慢性萎缩性胃炎患者(对照组)的IL-4基因内含子3的多态性,并分析其多态性与贲门癌、非贲门胃癌发病风险的相关性。结果贲门癌组、非贲门胃癌组的上消化道肿瘤(UGIC)家族史阳性个体的比例高于对照组。UGIC家族史可同时增加贲门癌和非贲门胃癌的发病风险(OR=2.738,95%CI:1.220～6.143;OR=2.452,95%CI:1.122～5.361)。IL-4内含子3的3种基因型(RP1.1、RP1.2、RP2.2)频率在贲门癌组、非贲门胃癌组和对照组中的总体分布差异有统计学意义(P<0.05);与RP1.1基因型比较,携带RP1.2基因型可增加非贲门胃癌的发病风险(OR=2.111,95%CI:1.172～3.803)。分层分析显示,与RP1.1基因型相比,RP1.2基因型能增加男性(OR=2.496,95%CI:1.235～5.047)和UGIC家族史阴性(OR=1.909,95%CI:1.020～3.573)非贲门胃癌的发病风险;而携带RP2.2基因型可使家族史阴性非贲门胃癌的发病风险降低(OR=0.496,95%CI:0.268～0.918)。结论 IL-4内含子3基因多态性可能是影响中国福建省非贲门胃癌发病风险的因素之一。Objective To investigate the correlation of intron 3 gene polymorphisms of interleukin-4 with genetic susceptibili- ties to gastric cardiac carcinoma and nongastric cardiac carcinoma in Fujian province of China. Methods Polymerase chain reaction- sequence specific primer（PCR-SSP） and Sanger sequencing were performed to determine the polymorphisms of intron 3 gene of inter- leukin-4 in 100 gastric cardiac carcinoma patients,130 nongastric cardiac carcinoma patients and 113 control subjects in Fujian prov- ince of China. Their correlation with the risk of gastric cardiac carcinoma and nongastric cardiac carcinoma was analyzed. Results The number of the subjects with family history of upper gastrointestinal cancer（UGIC） was significantly higher in gastric cardiac c, arci- noma group and nongastric cardiac carcinoma group than in control group（P 〈0. 05）. Family history of UGIC may increase the risk of developing gastric cardiac carcinoma and nongastric cardiac carcinoma （ OR = 2. 738,95% CI ： 1. 220-6. 143 ; OR = 2.452, 95% CI- 1. 122-5. 361 ）. The overall genotype distributions of intron 3 of interleukin-4 in gastric cardiac carcinoma group and nongastric cardiac carc, inoma group were significantly different from those in control group（P 〈0. 05）. Compared with individuals with RP1. 1 genotype, individuals with RP1.2 genotype had significantly higher risk in developing nongastric cardiac carcinoma（ OR = 2. 111,95% CI：1. 172- 3. 803）. When stratified for gender and family history of UGIC, compared with individuals with RP1.1 genotype, individuals with RP1.2 genotype in male group （OR = 2. 496,95% CI：1. 235-5. 047 ） and in the group without family history of U GIC （OR = 1. 909, 95% CI：1. 020-3. 573） had higher risk in developing nongastric cardiac carcinoma, and individuals with RP2.2 genotype in the group without tamily history of UGIC had lower risk in developing nongastric cardiac carcinoma（ OR = 0. 496,95% C1：0. 268-0. 918 ）. Con-clusion Intron 3 polymorphisms of i

关 键 词：白细胞介素4 基因多态性 贲门癌 非贲门胃癌 

分 类 号：R735.2[医药卫生—肿瘤]