FAT10过表达对HepG2肝癌细胞生长及凋亡的影响  

Effect of FAT10 over-expression on proliferation and apoptosis of HepG2 hepatocellular carcinoma cells

作　　者：高芸[1] 李少一[2] 商雪莹[1] 吕男男[1] 单忠艳[1,3] 滕卫平[1] 

机构地区：[1]中国医科大学附属第一医院内分泌研究所,辽宁沈阳110001 [2]中国医科大学附属第一医院内分泌科,辽宁沈阳110001 [3]中国医科大学附属盛京医院神经外科,辽宁沈阳110004

出　　处：《现代肿瘤医学》2013年第8期1674-1676,共3页Journal of Modern Oncology

基　　金：国家自然科学基金资助项目(No.81102471)

摘　　要：目的:探讨FAT10过表达对人肝癌细胞株HepG2生长和凋亡的影响。方法:采用脂质体法将FAT10质粒导入HepG2细胞,建立FAT10过表达的肝癌细胞系。Western blot检测细胞中FAT10表达水平,CCK-8细胞计数试剂盒,Annexin V凋亡试剂盒检测FAT10过表达对HepG2细胞生长和凋亡的影响。人类基因表达谱芯片检测FAT10下游与细胞增殖和凋亡相关基因的差异表达。结果:Western blot结果显示FAT10过表达细胞FAT10蛋白水平明显高于对照组,CCK-8检测显示FAT10过表达细胞增殖速度高于空载体组和对照组,而抗喜树碱诱导凋亡的能力FAT10过表达组较空载体组和对照组强。芯片分析显示,FAT10下游与细胞增殖和凋亡相关的若干基因表达有显著差异。结论:FAT10过表达可以促进HepG2细胞增殖和抗凋亡能力。Objective：To study the effect of FAT10 over-expression on proliferation and apoptosis of hepatocellular carcinoma cell line HepG2.Methods：Recombinant plasmid pcDNA3-FAT10 was trasfected into hepatocellular carcinoma cell line HepG2 by lipofectamine 2000.The efficiency of FAT10 over-expression was assessed by Western blot.The effect of FAT10 over-expression on proliferation and apoptosis was determined by CCK-8 and Annexin V,respectively.Results：Compared with the negative control and parental group,Western blot results demonstrated that the protein level of FAT10 was significantly increased.Over-expression of FAT10 in HepG2 cells significantly promote cellular proliferation and resistance against camptothecin-induced apoptosis.Genome-wide expression analyses revealed genes involved in proliferation and apoptosis were significantly differentially expressed in cells stably expressing FAT10 compared to control cells.Conclusion：Over-expression of FAT10 can significantly facilitate the growth and survival in HepG2 cells.

关 键 词：FAT10 肝癌 HEPG2 

分 类 号：R730.231[医药卫生—肿瘤] R735.7[医药卫生—临床医学]