IL-10、iNOS在幽门螺旋杆菌相关性胃炎及胃癌前病变组织中表达的相关性研究  

Correlation of Interleukin-10 and inducible nitric oxide synthase in Helicobacter pylori infected gastritis and precancerous lesions of gastric cancer

作　　者：张小艳[1] 王曙升 宋瑛[1] 徐俊荣[1] 杨震威[1] 

机构地区：[1]西安市中心医院消化科,陕西西安710003 [2]陕西省肿瘤医院,陕西西安710061

出　　处：《现代肿瘤医学》2013年第8期1830-1833,共4页Journal of Modern Oncology

摘　　要：目的:了解IL-10、iNOS在H.pylori相关性胃炎及胃癌前病变组织中的表达及相关性,为免疫治疗H.pylori相关性胃炎及预防胃癌的发生提供实验依据。方法:收集因各种症状于我院行胃镜检查并诊断为胃炎者110例,取胃窦部黏膜活检标本检测,其中符合实验研究条件74例。采用免疫组化方法检测74例标本中IL-10、iNOS蛋白的表达情况。结果:IL-10、iNOS在H.pylori(+)胃炎及胃癌前病变中的阳性表达率分别为49.15%、61.02%,与H.pylori(-)组的13.33%、20.00%相比,两种因子的阳性表达率均显著增加(P<0.05)。H.pylori(+)组,IL-10、iNOS在慢性浅表性胃炎中的阳性表达率分别为64.00%、44.00%,慢性萎缩性胃炎分别为38.10%、81.00%,肠化生及异型增生分别为38.46%、61.54%。IL-10在3组之间差异无显著性(P>0.05)。iNOS在慢性萎缩性胃炎组表达显著增高,与慢性浅表性胃炎组相比,差异显著(P<0.001),与肠化生及异型增生组相比差异无显著性(P>0.05)。H.pylori(+)组,IL-10与iNOS的表达呈显著负相关(r=-0.465,P<0.001)。结论:IL-10、iNOS两种因子与H.pylori感染相关。iNOS与胃癌的发生有关。是胃癌发生的早期事件,IL-10可能抑制了iNOS的过度表达及活性,在H.pylori相关胃炎及胃癌癌前病变中发挥保护作用。Objective：To investigate the correlation and expression of Interleukin-10（IL-10） and inducible nitric oxide synthase（iNOS） in Helicobacter pylori（H.pylori）-infected gastritis and precancerous lesions of gastric cancer（PLGC）,provide the experimental basis of immunotherapy of H.pylori-infected gastritis and preventing gastric cancer.Methods：Endoscopic biopsies were obtained from gastric antrum at endoscopy from 110 patients with gastritis diagnosed in our hospital.The samples of 74 patients fulfilled the criteria of study.The expression of IL-10 and iNOS was detected by immunohistothemistry.Results：The expression of IL-10 and iNOS in H.pylori positive gastritis and precancerous lesions of gastric cancer were 49.15% and 61.02%,which were significantly higher than those in H.pylori negative（13.33% vs 20.00%）（P 0.05）.In H.pylori positive group,the expression of IL-10 and iNOS in chronic superficial gastritis were 64.00% and 44.00% respectively,38.10% and 81.00% in chronic atrophic gastritis,38.46% and 61.54% in intestinal metaplasia and dysplasia group.This difference was not statistically significant in the expression of IL-10（P 0.05）.iNOS expression was higher in chronic atrophic gastritis than in chronic superficial gastritis（P 0.05）,but there was no prominent difference compared with intestinal metaplasia and dysplasia（P 0.05）.In H.pylori positive group,there was a negative correlation between IL-10 and iNOS（r =-0.465,P 0.001）.Conclusion：The expressions of IL-10 and iNOS are associated with H.pylori infection.IL-10 may have a protective action by inhibiting the overexpression of iNOS,which has correlation with gastritis and gastric cancer.

关 键 词：白细胞介素-10 诱导型一氧化氮合酶 幽门螺杆菌 胃炎 胃癌前病变 

分 类 号：R735.2[医药卫生—肿瘤]