Tg-SwDI/B小鼠空间记忆能力与脑内β淀粉样蛋白分布的研究  

The Association of Spatial Memory Function with Distribution of Amyloid β Protein in the Brain of Tg-SwDI/B Mice

作　　者：甘莉[1,2] 闵喆[1] 熊永洁[1] 王倩[1] 方瑜[1] 张苏明[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院神经内科,武汉430030 [2]南昌大学第二附属医院神经内科,南昌330006

出　　处：《神经损伤与功能重建》2013年第4期243-246,共4页Neural Injury and Functional Reconstruction

摘　　要：目的:观察Tg-SwDI/B小鼠空间记忆能力与脑内β淀粉样(Aβ)蛋白分布的情况。方法:12月龄纯合子Tg-SwDI/B小鼠和野生型C57BL/6各20只,雌雄比例为1∶1;通过八臂迷宫检测小鼠的空间记忆能力,硫磺素-S染色和GFAP免疫荧光染色显示Aβ及星形胶质细胞在小鼠脑内的分布特点。结果:Tg-SwDI/B小鼠空间记忆功能明显受损,表现为长期记忆功能下降,脑内有大量纤维化Aβ沉积在血管或其周围,并且可见GFAP阳性星形胶质细胞,两者均在海马和丘脑区域更为多见。同月龄野生型小鼠脑内未见纤维化Aβ沉积,存在少量GFAP阳性星形胶质细胞,但数量明显少于Tg-SwDI/B小鼠。结论:Tg-SwDI/B小鼠的空间记忆功能受损,可能与海马区域血管相关性纤维化Aβ和活化的星形胶质细胞增生相关。Objective： To observe the spatial memory and it＇ s relationship with distribution of amyloid-β protein deposition in the Tg-SwDI/B mice brain. Methods： There were 20 homozygous Tg-SwDUB and wild-type C57BL/6 male and female mice at the age of 12 months individually. Radial ann maze was used to test the spatial memory of all the mice, and thioflavine-S and glia fibrillary acidic protein （GFAP） were stained to study the distribution orAβ and astrocytes. Results： The spatial memory of Tg-SwDI/B mice was impaired obviously at the age of 12 months. Numerous fibrillar AI3 was accumulated in and around microvessels, and GFAP-positive reactive astrocytes were significantly elevated in all brain regions of Tg-SwDI/B mice, particularly in the hippocampal and thalamic regions. No deposits of fibrillar Aβ was observed in the brain of wild type C57BL/6 mice. Few GFAP-positive astrocytes were observed compared with those in Tg-SwDI/B mice. Conclusion： Spatial memory of Tg-SwDI/B mice was impaired, the microvascular fibrillar Aβ deposit and activated astrocytes proliferation in hippocampal region perhaps associated with the defect of spatial memory.

关 键 词：Tg—SwDI B小鼠 Β淀粉样蛋白 纤维化 星形胶质细胞 空间记忆受损 

分 类 号：R741[医药卫生—神经病学与精神病学] R741.02[医药卫生—临床医学]