Degradable PRGD/PDLLA/β-TCP/NGF composites promote differentiation and regulate gene expression in rat pheochromocytoma cells  

作　　者：QIU Tong YU Chang YAN QiongJiao LI ShiPu 

机构地区：[1]Biomedical Materials and Engineering Center, Wuhan University of Technology [2]State Key Laboratory of Advanced Technology for Materials Synthesis and Processing,Wuhan University of Technology

出　　处：《Chinese Science Bulletin》2013年第24期2979-2983,共5页

基　　金：supported by the National Basic Research Program of China (2011CB606205)

摘　　要：In this study, we synthesized degradable PRGD/PDLLA/β-TCP/NGF composites to facilitate neuronal repair. To this end, we (1) examined the release of nerve growth factor (NGF) from the composites, (2) evaluated the differentiation status of the cells and (3) address how transcriptional activity may regulate the differentiation mechanism of these cells. NGF content was determined using enzyme-linked immunosorbent assay, while the cellular mRNA expression was examined by real-time PCR analysis. Our results indicated that NGF release was robust during the first 10 days and then stabilized at a lower level thereafter. Treatment of PC12 cells with the extract of the NGF-embedded composites induced the formation of neurites and, in some cases, net-like neurites. Analysis of the expression level of differentiation-related genes, such as TrkA, VGF, Rab1, GAP43 and β-tubulin Ⅱ, were significantly up-regulated. These findings suggest that these composites might be a suitable delivery system for growth factors like NGF that can be used to facilitate neuronal repair after injury.In this study, we synthesized degradable PRGD/PDLLA/β-TCP/NGF composites to facilitate neuronal repair. To this end, we （1） examined the release of nerve growth factor （NGF） from the composites, （2） evaluated the differentiation status of the cells and （3） address how transcriptional activity may regulate the differentiation mechanism of these cells. NGF content was determined using enzyme-linked immunosorbent assay, while the cellular mRNA expression was examined by real-time PCR analysis. Our results indicated that NGF release was robust during the first 10 days and then stabilized at a lower level thereafter. Treatment of PC12 cells with the extract of the NGF-embedded composites induced the formation of neurites and, in some cases, net-like neurites. Analysis of the expression level of differentiation-related genes, such as TrkA, VGF, Rabl, GAP43 and β-tubulin H, were signifi- cantly up-regulated. These findings suggest that these composites might be a suitable delivery system for growth factors like NGF that can be used to facilitate neuronal repair after injury.

关 键 词：细胞分化 复合材料 PDLLA 基因表达 合成降解 嗜铬细胞瘤 神经生长因子 神经元损伤 

分 类 号：Q421[生物学—神经生物学]