Myofibrillogenesis regulator-1 promotes cell adhesion and migration in human hepatoma cells  

作　　者：ZHAO WuLi HE HongWei REN KaiHuan ZHANG Hao CHEN Yi SHAO RongGuang 

机构地区：[1]Ministry of Health Key Laboratory of Antibiotic Bioengineering, Department of Oncology, Institute of Medicinal Biotechnology, Peking Union Medical College, Chinese Academy of Medical Sciences

出　　处：《Chinese Science Bulletin》2013年第24期3007-3014,共8页

基　　金：supported by the National Basic Research Program of China (2009CB521807);the National Natural Science Foundation of China (30772583, 30900237);National S&T Major Special Project on Major New Drug Innovation (2012ZX09301002-001)

摘　　要：Myofibrillogenesis regulator-1 (MR-1) is a gene overexpressed usually in many human cancers. However, the effects of MR-1 on cell proliferation, adhesion, migration and genome-wide gene regulation are still unclear. In this study, a human hepatoma cell line that highly overexpresses MR-1, BEL-7402/MR-1 cells was established. While the high expression of MR-1 did not promote cell proliferation, it significantly increased cell spreading, adhesion and migration compared with control cells. A total of 147 genes were regulated by MR-1 expression, 46 genes were down-regulated and 101 genes were up-regulated by MR-1 overexpression. Many of these genes were related to cell adhesion, cytoskeletal regulation, MAPK signaling, and cell cycle related pathways. Western blot analysis further confirmed the regulation of pathways associated with migration by MR-1. These results suggest that MR-1 is involved in the regulation of cancer cell adhesion, migration and related gene expression.Myofibrillogenesis regulator-1 （MR-l） is a gene overexpressed usually in many human cancers. However, the effects of MR-1 on cell proliferation, adhesion, migration and genome-wide gene regulation are still unclear. In this study, a human hepatoma cell line that highly overexpresses MR-l, BEL-7402/MR-1 cells was established. While the high expression of MR-1 did not promote cell proliferation, it significantly increased cell spreading, adhesion and migration compared with control cells. A total of 147 genes were regulated by MR-1 expression, 46 genes were down-regulated and 101 genes were up-regulated by MR-1 overexpres- sion. Many of these genes were related to cell adhesion, cytoskeletal regulation, MAPK signaling, and cell cycle related pathways. Western blot analysis further confirmed the regulation of pathways associated with migration by MR-1. These results suggest that MR-1 is involved in the regulation of cancer cell adhesion, migration and related gene expression.

关 键 词：人肝癌细胞株 迁移 黏附 人类 基因表达调控 调节器 细胞粘附 BLOT分析 

分 类 号：R735.7[医药卫生—肿瘤]