经典激活巨噬细胞促进乳腺癌细胞贴壁和非贴壁克隆形成  

Classically activated macrophages promote anchorage-dependent and anchorage-independent clone formation of breast cancer cells

作　　者：陈静琦[1] 朱必胜[1] 侯开连[1] 

机构地区：[1]广州医学院第二附属医院肿瘤科,广东广州510260

出　　处：《实用肿瘤杂志》2013年第4期364-366,共3页Journal of Practical Oncology

基　　金：国家自然科学基金(81272900);广州市属高校科技计划资助项目(08A080)

摘　　要：目的探讨经典激活巨噬细胞(M1)对乳腺癌细胞贴壁和非贴壁克隆形成的影响,进一步阐明M1促乳腺癌进展的作用。方法用密度梯度离心法,从健康成人外周血中分离单个核细胞,体外诱导M1。在乳腺癌细胞与巨噬细胞的无血清共培养体系中,做平板克隆实验;在M1-乳腺癌细胞软琼脂培养体系中,做非贴壁克隆形成实验,检测M1对乳腺癌贴壁克隆和非贴壁克隆形成的影响。结果在M1的作用下,乳腺癌SK-3rd细胞贴壁克隆和非贴壁克隆形成的能力均增强(P<0.001)。结论 M1促进乳腺癌细胞贴壁克隆和非贴壁克隆的形成。Objective To investigate the effect of activated microphages （ M1 ） on the clone formation of breast cancer cells. Methods Mononuclear cells were isolated from peripheral blood of adult normal subjects by density gradient centrifugation, and M1 was induced in vitro. In the serum-free co-cuhure system of breast cancer cells and macrophages, the flat cloning assay was performed and in soft agar culture system of Ml-breast cancer cells, anchorage-independent clone formation assay was performed. Results The anchorage-dependent and anchorage-independent clone formation of SK-3rd breast cancer cells was induced by M1 （ P 〈 0. 001 ）. Conclusion M1 promotes anchorage-dependent and anchorage-independent clone formation of breast cancer cells.

关 键 词：乳腺肿瘤 巨噬细胞 药物作用 细胞增殖 克隆细胞 细胞 培养的 

分 类 号：R737.9[医药卫生—肿瘤]