微小核糖核酸-32对胃癌细胞增殖与迁移行为的影响  

Effect of microRNA-32 on the proliferation and migration of gastric cancer cells

作　　者：蒯小玲[1] 宋梦娇[1] 俞智华[1] 张健锋[1] 陈晓奇[1] 张弘[1] 毛振彪[1] 

机构地区：[1]南通大学附属医院消化科,江苏省南通市226001

出　　处：《中华消化杂志》2013年第7期465-469,共5页Chinese Journal of Digestion

摘　　要：目的探讨微小核糖核酸-32（miRNA-32）转染对胃癌细胞生物学行为的影响及其作用机制。方法应用脂质体法分别将miRNA-32类似物、miRNA-32抑制物和空质粒瞬时转染到胃癌细胞SGC-7901，分为miRNA-32类似物转染组、miRNA-32抑制物转染组、空质粒转染组、未转染组。荧光显微镜观察绿色荧光蛋白的表达，实时荧光定量PCR法检测miRNA-32mRNA的表达，CCK-8法检测细胞增殖能力，划痕实验、Transwell小室检测各组细胞体外迁移能力。统计学处理采用单因素方差分析。结果与未转染组、空质粒转染组相比，miRNA-32类似物转染组mRNA表达（相对定量值-2．327）明显上调，miRNA-32抑制物转染组mRNA表达（相对定量值-0．402）明显下调，差异有统计学意义（F=11．238，P〈0．05）。miRNA-32类似物转染组24h划痕宽度为（61．39±2．21）“m，miRNA-32抑制物转染组为（29．97±0．66）／xm，miRNA-32抑制物转染组细胞迁移距离较miRNA-32类似物转染组远，差异有统计学意义（F=9．371，P〈0．05）。转染48h后，miRNA-32类似物转染组穿膜细胞数较未转染组明显减少，分别为（16．93±4．63）个和（93．93±7．09）个，差异有统计学意义（F=6．853，P％0．05）。转染后48、72h，miRNA-32类似物转染组细胞生长抑制率分别为（43．474±18．636）％和（45．0504-23．764）％，细胞出现明显生长抑制（F=7．986、8．635，P=0．028、0．032）。结论上调miRNA-32表达可明显抑制人胃癌细胞SGC-790l的生长，抑制其迁移能力。Objective To explore the effect of microRNA-32 （miRNA-32）on the biological behaviors of gastric cancer cell and its mechanism. Methods Gastric cancer cell line SGC-7901 cells were transiently transfected with miRNA-32 analogue, miRNA-32 inhibitor and empty plasmid vectors by lipofectamine and divided into analogue transfection group, inhibitor transfection group, empty plasmid transfection group and non-transfection group. The expression of green fluorescent protein was observed under fluorescent microscopy. The expression of miRNA 32 at mRNA level was detected by quantificational real-time polymerase chain reaction. The cell proliferation was evaluated by CCK-8 assay. The cell migration ability was measured by scratch test and Transwell chamber assays. The data were analyzed by one-way ANOVA. Results Compared with empty plasmid transfection group and non transfection group, the expression of miRNA-32 mRNA of miRNA-32 analogue transfection group （relative quantitative value： 2. 327） was significantly up-regulated and that of miRNA-32 inhibitor transfection group （relative quantitative value： 0. 402） was significantly down-regulated （F= 11. 238, P〈 0. 05）. The width of scratch of miRNA-32 analogue transfection group was （61. 39 ± 2.21） μm at 24 hours; miRNA-32 inhibitor transfection group was （29.97±0.66） μm. The migration distance of inhibitor transfection group was far than that of analogue transfeetion group （F= 9. 371, P〈0.05）. A{ter transfection for 48 hours, the cell number of migrated ceils of analogue transfection group was significantly less than that of non-transfection group, which was 16.93±4.63 and 93.93±7.09, respectively （F=6.853,P〈0.05）. After transfection for 48 hours and 72 hours, the cell growth inhibiting rate of miRNA-32 analogue transfection group was （43. 474± 18. 636） % and （45. 050±23. 764） %, respectively, the cell growth was significantly inhibited （F 7. 986 and 8. 635, P=0. 028 and 0. 032）. Conclusion The cell growth and migr

关 键 词：胃肿瘤 细胞增殖 细胞运动 微RNAS 

分 类 号：R735.2[医药卫生—肿瘤]