盐霉素对人肺腺癌A549细胞株生物学特性的影响  

Effects of Salinomycin on the biological characteristics of lung adenocarcinoma cell line AS49 cells

作　　者：石俊杰[1] 王哲[1] 李阳 张翼翔[1] 刘晶 顾春东[1] 

机构地区：[1]大连医科大学附属第一医院胸外科肺癌诊疗基地,116011 [2]中英再生医学中心

出　　处：《中华实验外科杂志》2013年第8期1592-1594,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（81173453）;大连市科技计划重大项目（2012E15SF141）

摘　　要：目的观察盐霉素对人肺腺癌A549细胞株生物学特性的影响。方法无血清培养A549干细胞，进行划痕、Transwell和细胞计数实验，运用流式细胞仪检测细胞侧群比例，采用Westernblot检测盐霉素对转录因子Oct4表达的影响。结果空白对照组、30mg／L、60mg／L盐霉素组迁移距离分别为70、40、10um，侵袭细胞数目分别为（32．3±2．5）、（21．4±1．8）、（1．3±0．3）个／HP，细胞存活率分别为98％、74％、50％；60mg／L组、30mg／L组、空白对照组、利血平阻断组侧群比例分别为3．4％、10．5％、12．5％、0．7％。Westernblot示随着盐霉索浓度增加，Oct4的表达量下降。结论盐霉素对人肺腺癌细胞株A549生物学特性具有重要影响。Objective To investigate the effects of Salinomycin on the biological characteristics of lung adenocarcinoma cell line A549 cells. Methods The scratch test, Transwell assay and cell counting kit-8 assay were applied to study the influence of Salinomycin on biological characteristics of lung adenocarci- noma A549 cells. Flow cytometry was used to analyze the proportion of side population cells. Western blot- ting was used to detect the expression of Oct4 protein in A549 cells treated with Salinomycin. Results In blank control group, 30 mg/L Salinomycin group and 60 mg/L Salinomycin group, migration distance was 70, 40 and 10 μm, the number of invasive cells was （32. 3 ±2. 5）, （21.4 ±1.8） and （ 1.3 ±0. 3）/HP, and cell survival rate was 98%, 74% and 50%, respectively. The proportion of side population cells in 60 mg/L Salinomycin group, 30 mg/L Salinomycin group, blank control group and reserpine blocking group was 3.4%, 10. 5%, 12. 5% and 0. 7% respectively. Western blotting revealed that the Oct4 expression was de- creased with increasing Salinomycin. Conclusion Salinomycin has an important impact on the biological characteristics of lung adenocarcinoma cell line A549. Salinomycin might become a new effective drug for the treatment of lung adenocarcinoma.

关 键 词：肺腺癌 盐霉素 干细胞 转录因子Oct4 

分 类 号：R734.2[医药卫生—肿瘤]