蛋白酪氨酸磷酸酶-3通过诱导肿瘤性巨噬细胞钙离子依赖性钾离子通道表达增强LoVo细胞侵袭性  

Protein tyrosine phosphatase 3 improves the invasion of LoVo cells through induceing Ca2＋ -activa- ted K ＋ channels 4 of tumor associated macrophages

作　　者：许鹤洋[1] 刁飞宇[1] 曾育杰[1] 张旸[1] 来伟[1] 褚忠华[1] 

机构地区：[1]中山大学孙逸仙纪念医院胃肠外科,广州510288

出　　处：《中华实验外科杂志》2013年第8期1666-1667,共2页Chinese Journal of Experimental Surgery

基　　金：广东省自然科学基金自由申请项目（10151008901000071）;广东省自然科学基金资助项目（S2012010009161）;广东省社会发展计划基金资助项目（201211050600014）

摘　　要：目的观察结肠癌肿瘤微环境中肿瘤细胞与巨噬细胞（M2）相互作用及对肿瘤细胞功能学的改变。方法将转入蛋白酪氨酸磷酸酶．3（PRL-3）的LoVo细胞和M2细胞模拟肿瘤微环境进行共培养，通过Westernblot检测M2细胞钙离子依赖性钾离子通道（KCNN4）蛋白表达，并检测LoVo细胞侵袭性的改变。结果Westernblot检测示PRL-3能通过共培养后诱导M2细胞KCNN4蛋白表达升高，而未作共培养的M2细胞KCNN4蛋白表达未升高。此外，经过共培养后LoVo细胞侵袭性升高（3367±135比1442±89，P〈0．05），而在阻断KCNN4蛋白表达后，LoVo细胞侵袭性降低（1388±87比2893±163，P〈0．05）。结论PRL-3在结肠癌肿瘤微环境中通过提高KCNN4表达从而增强LoVo细胞的侵袭性。Objective To investigate the interaction between colon cancer cells and tumor associ- ated cells （M2） , and it＇ s influence to tumor cells＇ function. Methods LoVo cells [ transfeeted with pro- tein tyrosine phosphatase 3 （PRL-3） ] and M2 cells were coeultured, Western blot were used to examine Ca2＋ -activated K＋ channels （KCNN4） expression of M2 cells. And the invasion of LoVo cells also he de- tected. Results Used by Western blotting it is found out that KCNN4 of M2 cells is improved after eoeul- tured with PRL-3 LoVo cells. Besides, eocuhure also improves the invasion of LoVo cells（3367 ± 135 vs. 1442 ± 89, P 〈 0. 05 ）. When KCNN4 expression was blocked, the invasion of LoVo cells is reduced （ 1388 ± 87 vs. 2893 ± 163, P 〈 0. 05 ）. Conclusion PRL-3 improves the invasion of LoVo cells through upregulating KCNN4 of M2 cells in the colon cancer microenvironment.

关 键 词：蛋白酪氨酸磷酸酶-3 肿瘤性巨噬细胞 钙离子依赖性钾离子通道 

分 类 号：R735.35[医药卫生—肿瘤]