肺癌抑癌基因1对人骨肉瘤细胞株MG63生长和增殖的影响  

The effect of tumor suppressor in lung cancer-1 gene on growth and proliferation of human osteo- sarcoma cell line MG63

作　　者：覃莉[1] 林阳[2] 陈文坚[2] 陈安民[2] 祝文涛[2] 

机构地区：[1]武汉市第一医院皮肤科,430022 [2]华中科技大学同济医学院附属同济医院骨科

出　　处：《中华实验外科杂志》2013年第8期1704-1706,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金青年基金资助项目（81202121）;湖北省自然科学基金资助项目（2010CDB09302）;武汉市青年科技晨光计划（200950431209）

摘　　要：目的观察肺癌抑癌基因1（TSLC1l）对人骨肉瘤细胞株MG63的生长和增殖能力的影响。方法将稳定表达TSLC1的人骨肉瘤MG63细胞株M8T设为研究对象，转染空载体的骨肉瘤MG63细胞株M8P设为对照组，人骨肉瘤细胞株MG63设为空白组。噻唑蓝（MTT）法检测M8T细胞增殖；FACSort流式细胞仪检测细胞周期变化及细胞凋亡；将2×10^7个瘤细胞皮下注射裸鼠，观察体内成瘤的影响。结果稳定转染TSLC1的实验组M8T细胞株与对照组及空白组细胞比较，其细胞增殖能力下降，生长速度明显受到抑制；M8T细胞G0～G1期细胞数为（63．76±2．78）％，生长周期出现了G0-G0期阻滞，细胞凋亡总数为（28．45±0．65）个，显著增加（P〈0．1）；裸鼠皮下成瘤于皮下注射后3周开始形成。结论TSLC1能明显抑制骨肉瘤MG63细胞的生长和增殖。Objective To elucidate the effect of tumor suppressor in lung cancer-1 （TSLC1） on growth and proliferation of human osteosarcoma cell line MC,63 which stably expressed TSLC1 gene named M8T. Methods Human osteosareoma cell subline MST was stably transfected with exogenous gene TSLC1. Cell growth was analyzed with methyl thiazol tetrazolium （MTT） assay. FACSort flow eytometry a- nalysis was performed to assess the cell cycle distribution and apoptosis. 2× 10^7 cells were subcutaneous injected of nude mice. Mice were sacrificed started on week 6 after injection, and tumors were evaluated un- der both macroscopic and microscopic observation with hematoxylin and eosin （HE） staining. Results The growth of TSLCl-transfected cells MST was significantly suppressed in vitro, displaying that the amount of G0-G1 cells increased to （63.76 ± 2. 78 ） % and the amount of S phase cells decreased significantly, which suggested a G0-G1 cell cycle arresting. The total number of cell apoptosis of M8T is 28.45 ± 0. 65. The M8T cells proliferation were significantly reduced in vitro in comparison to the control and blank groups. Moreover, tumorigenicity of M8T cells was suppressed in vivo, subcutaneous tumor formation of nude mice occurred 3 weeks later and less than the control and blank groups. Conclusion The ability of growth and proliferation of human osteosarcoma cell subline MST was significantly suppressed by TSLC1 both in vitro and in vivo.

关 键 词：肺癌抑癌基因1 骨肉瘤 增殖 

分 类 号：R734.2[医药卫生—肿瘤]