石油降解菌产表面活性剂的条件优化  

Optimization of conditions for oil-degrading bacteria producing surfactant

作　　者：高小朋[1] 姜钊[1] 高秀梅[1] 王跃[1] 任桂梅[1] 贺晓龙[1] 

机构地区：[1]延安大学生命科学学院,延安716000

出　　处：《环境工程学报》2013年第8期3244-3248,共5页Chinese Journal of Environmental Engineering

基　　金：陕西省科技统筹创新工程计划项目(2011KTCL01-18);延安市科技计划项目(2011ks-38);陕西省教育厅科研计划项目(12JK0845);延安大学大学生科技创新训练计划项目(D2011-40)

摘　　要：为了了解陕北石油降解菌产表面活性剂的情况,对实验菌株CT-6以原油为唯一碳源时产表面活性剂的排油活性、表面张力和乳化性能进行了研究,并对实验菌株产表面活性剂的条件进行了优化。结果表明,排油圈直径达到8.0cm,表面张力降低到33 mN/m,乳化指数达89.9%,石油降解率达到68.0%;优化后的条件为:接种量10%、pH为8、温度28℃、转速220 r/min,该条件下,排油圈直径增加了13.1%,7 d时乳化指数达到93.0%。We preliminarily studied the situation of the petroleum degrading bacteria CT-6 that can produce the surfactant in northern of Shanxi,identified the oil extraction,surface tension and emulsifiability when the CT-6 used crude oil as the only carbon source,and optimized the conditions of the strain that produce the surfanctant.The results reveal that oil displacement diameter is 8.0 cm,the surface tension reduces to 33 mN/m,EI-24 is 89.9%,oil degradation rate reaches 68.0%.The optimized conditions are inoculum size 10%,pH 8,28℃,220 r/min,and under the conditions,the oil spreading diameter increases by 13.1% and emulsifying properties reach to 93.0% by 7 d.

关 键 词：石油 降解 表面活性剂 

分 类 号：X172[环境科学与工程—环境科学]