EZH2mRNA在结直肠癌组织中的表达及其临床意义  

Expression and Clinical Value of EZH2 mRNA in Colorectal Cancer Tissues

作　　者：吕艳锋[1] 韩冰冰[2] 禹化龙[1] 王建新[1] 

机构地区：[1]山东大学第二医院肛肠外科,山东济南250033 [2]山东中医药大学微循环实验室,山东济南250014

出　　处：《中国普外基础与临床杂志》2013年第7期752-755,共4页Chinese Journal of Bases and Clinics In General Surgery

基　　金：山东省自然科学基金(项目编号:Y2008C120);山东济南青年科技明星计划(项目编号:20090208)~~

摘　　要：目的探讨zeste基因增强子同源物2(EZH2)mRNA在结直肠癌组织中的表达及其与结直肠癌临床病理特征的关系。方法采用PCR方法检测了27例结直肠癌患者的癌组织、癌旁组织和正常黏膜上皮组织中EZH2 mRNA的表达情况,并分析其与结直肠癌临床病理特征的关系。结果结直肠癌组织、癌旁组织及正常黏膜上皮组织中EZH2 mRNA的表达水平分别为3.01±1.29、1.20±0.69及0.87±0.37,其在结直肠癌组织的表达水平最高(P<0.01),而在癌旁组织和正常黏膜上皮组织中的表达差异无统计学意义(P=0.403)。结直肠癌组织中EZH2 mRNA的表达与其TNM分期(P=0.002)和组织分化程度(P=0.005)均有关,与年龄(P=0.388)、性别(P=0.963)、肿瘤直径(P=0.579)、组织学类型(P=0.945)、肿瘤位置(P=0.611)及淋巴结转移(P=0.449)均无关。结论 EZH2 mRNA在结直肠癌组织中呈高表达,可能在结直肠癌的发生和发展过程中起关键作用,可作为判断结直肠癌恶性程度及进程的参考指标之一。Objective To investigate the expression of enhancer of zeste homolog 2（EZH2）mRNAin colorectal cancer tissues,and to explore the relationship with clinicopathological features of it.Methods PCR was used to examine the expression of EZH2 mRNA in colorectal cancer tissues,paracancerous tissues,and normal epithelium tissues of 27 patients with colorectal cancer.Meanwhile,the relationship between the expression of EZH2 mRNA and clinicopathological features of colorectal cancer were analyzed.Results Expression level of EZH2 mRNA in colorectal cancer tissues,paracancerous tissues,and normal epithelium tissues were 3.01±1.29,1.20±0.69,and 0.87±0.37 respectively,and the expression value of colorectal cancer tissues at the top（P 0.01）,but there were no significant difference between the paracancerous tissues and normal epithelium tissues（P=0.403）.Expression of EZH2 mRNA were related to TNM stages（P=0.002）and degree of differentiation（P=0.005）,but were not related to age（P=0.388）,gender（P=0.963）,diameter of tumor（P=0.579）,histological type（P=0.945）,location of tumor（P=0.611）,and lymph node metastasis（P=0.449）.Conclusions EZH2 mRNA expresses highly in colorectal cancer tissues,so it may play an essential role in the emergence and development of colorectal cancer.EZH2 mRNA can be used as one of the referenced indexes to determine the malignant degree and process of colorectal cancer.

关 键 词：zeste基因增强子同源物2 结直肠癌 基因 表达 

分 类 号：R735.34[医药卫生—肿瘤]