人胃癌耐药细胞株中锌指蛋白139和多药耐药基因表达的关系及意义  

Relationship between ZNF139 and multidrug resistance(MDR) related genes in SGC7901 and SGC7901/ADR cell lines

作　　者：李勇[1] 赵群[1] 檀碧波[1] 范立侨[1] 刘庆伟[1] 焦志凯[1] 赵雪峰[1] 郝英杰[1] 

机构地区：[1]河北医科大学第四医院外三科,河北石家庄050011

出　　处：《中国癌症杂志》2013年第7期493-498,共6页China Oncology

基　　金：国家自然科学基金(No:81072033);河北省自然科学基金(No:C2010000619);河北省普通高校强势特色学科资助项目(No:冀教高[2005]52);河北省卫生厅科研基金资助项目(No:20110460)

摘　　要：背景与目的:研究发现锌指蛋白139(ZNF139)在胃癌中表达异常,但ZNF139与胃癌的多药耐药性(multidrugresistance,MDR)关系尚不明确。本研究检测了人胃癌细胞株SGC7901和耐药细胞株SGC7901/ADR中ZNF139和MRP-1、MDR1/P-gp、GST-π的mRNA和蛋白表达情况,并对其表达关系和意义进行分析。方法:体外培养SGC7901、SGC7901/ADR,逆转录-聚合酶连反应(reverse transcription-polymerase chain reaction,RT-PCR)检测两种细胞ZNF139和MRP-1、MDR1/P-gp、GST-πmRNA表达情况,采用蛋白质印迹法(Western blot)法检测各基因蛋白表达变化情况。合成针对ZNF139的小干扰RNA(siRNA-ZNF139)重组质粒并转染SGC7901/ADR,检测转染前后SGC7901/ADR中MRP-1、MDR1/P-gp、GST-π表达的变化。结果:SGC7901和SGC7901/ADR两种细胞中均有ZNF139、MRP-1、MDR1/P-gp、GST-πmRNA和蛋白阳性表达,SGC7901/ADR细胞中ZNF139、MRP-1、MDR1/P-gp、GST-πmRNA和蛋白表达均明显高于SGC7901细胞(P<0.05)。转染siRNA-ZNF139后SGC7901/ADR细胞中ZNF139表达明显受到抑制,MRP-1、MDR1/P-gp、GST-π表达也明显降低(P<0.05)。结论:ZNF139通过上调MRP-1、MDR1/P-gp、GST-π基因表达而参与胃癌多药耐药形成。Background and purpose： It was reported that zinc finger protein 139 （ZNF139） was expressed aberrantly in gastric cancer. But the relationship between ZNF139 and multidrug resistance （MDR） of gastric cancer is still not clear. The purpose of this research was to investigate the expressions and significance of ZNF139, MRP-1, MDR1/P-gp, GST-π in human gastric carcinoma cell lines SGC7901 and SGC7901/ADR. Methods： The expressions of ZNF139, MRP-1, MDR1/P-gp, GST-π were determined with RT-PCR and Western blot in SGC7901 and SGC7901/ADR cell lines. Then siRNA recombinant plasmid of targeting ZNF139 gene was constructed and imported into gastric cancer cell line SGC7901/ADR, and the expressions of MRP-1, MDR1/P-gp, GST-π were tested simultaneously. Results： The expressions ofZNF139, MRP-1, MDR1/P-gp, GST-π were higher in SGC7901/ADR than in SGC7901（P〈0.05）. ZNF 139 was inhibited obviously after siRNA-ZNF 139 was transfected into SGC7901/ADR, and expression ofMRP-1, MDR1/P-gp, GST-π decreased（P〈0.05）. Conclusion： ZNF139 may be invovled in multidrug resistance （MDR） of gastric cancer by up-regulating MRP-1, MDR 1/P-gp and GST-π.

关 键 词：锌指蛋白139 胃癌 多药耐药性 

分 类 号：R735.2[医药卫生—肿瘤]