CD13^+CD133^+和CD13_-CD133_-肝癌细胞的生物学差异及临床意义  

The biological difference between CD13^+CD133^+ and CD13_-CD133_- liver cancer cells and its clinical significance

作　　者：金世龙[1] 黄中荣[1] 陈华[1] 余天雾[1] 曹洪[1] 龙运全[1] 周健[1] 李鹤[1] 苟毅[1] 李远[1] 廖娟[1] 

机构地区：[1]重庆医科大学附属永川医院肝胆外科,重庆402160

出　　处：《解放军医学杂志》2013年第8期661-664,共4页Medical Journal of Chinese People's Liberation Army

摘　　要：目的比较HuH7细胞系CD13+CD133+和CD13-CD133-肝细胞癌(HCC)细胞的生物学差异,并探讨其临床意义。方法通过对CD13+CD133+HCC细胞增殖情况、细胞周期、培养10d分化情况、裸鼠体内成瘤能力,以及对5-FU和吡柔比星等化疗药物的敏感性等生物学特征的研究,分析CD13+CD133+HCC细胞亚群的临床意义。结果 CD13+CD133+HCC细胞增殖速度明显快于CD13-CD133-HCC细胞,78.45%的CD13+CD133+HCC细胞处于G0/G1期,2.19%处于G2/M期,19.36%处于S期;62.18%的CD13-CD133-HCC细胞处于G0/G1期,11.88%处于G2/M期,25.95%处于S期。在裸鼠体内,1×103个CD13+CD133+HCC细胞即可成瘤,而1×105个CD13-CD133-HCC才可成瘤。CD13+CD133+HCC细胞对5-FU和吡柔比星具有抵抗特性,而其他3种亚群较易被杀灭。FACS分选的CD13+CD133+HCC和CD13-CD133-HCC细胞经过10d培养后,CD13+CD133+HCC亚群的CD13、CD133标志表达与HuH7细胞系相近,而CD13-CD133-HCC细胞不具有此能力。结论 CD13+CD133+HCC细胞具有肿瘤干细胞的特征,可能与临床肝癌复发和转移有关,是临床治疗过程中尤其需要杀灭的细胞。Objective To compare the biological difference between CD13＋CD133＋ and CD13-CD133-hepatocellular carcinoma（HCC） cells in HuH7 cell line and its clinical significance.Methods The status of proliferation,phase of the cell cycle,tumor formation in vivo,differentiation,and their chemoresistance to 5-FU and pirarubicin of CD13＋CD133＋ and CD13-CD133HCC cells were studied to analyze the clinical implication of CD13＋CD133＋HCC cell subset.Results The proliferation rate of CD13＋CD133＋HCC cells was signiicantly higher than that of CD13-CD133-HCC cells.he cell-cycle phase study showed that 78.45% of the CD13＋CD133＋HCC cells were in the G0/G1 phase,2.19% in G2/M phase,and 19.36% in S phase,while 62.18% CD13-CD133HCC cells were in the G0/G1phase,11.88% in G2/M phase,and 25.95% in S phase.Limiting dilution analysis of HuH7 cells revealed that 1×103CD13＋CD133＋cells could form the tumor,while 1×105CD13-CD133-cells did.CD13＋CD133＋cells showed chemoresistance to 5-FU and pirarubicin,while other three subsets succumbed to the drugs.Conclusion CD13＋CD133＋cancer cells in HuH7 showed the characteristics of cancer stem cells（CSCs）,which might contribute to the relapse and metastasis of liver cancer,and they may be the main target for chemotherapy in human liver cancer.

关 键 词：肝肿瘤 肿瘤干细胞 抗原 CD13 抗原 CD133 

分 类 号：R735.7[医药卫生—肿瘤]