Fingerprint analysis of Cirsium japonicum DC.using high performance liquid chromatography  

作　　者：Hongli Ge Muhetar Turhong Munire Abudkrem Yuhai Tang 

机构地区：[1]School of Science,Xi'an Jiaotong University [2]Kashi Teachers'College

出　　处：《Journal of Pharmaceutical Analysis》2013年第4期278-284,共7页药物分析学报（英文版）

基　　金：supported by the Main Program of Autonomous Region College Scientific Research Program (XJEDU2008145)

摘　　要：In many areas of China Cirsium setosum is used as Cirsiumjaponicum DC. Although the two herbs have similar appearance and many similar compounds, they are totally different medicinal material, and have different pharmacodynamic actions. The fingerprint spectrum can be a good tool to distinguish the two herbs and control the quality of Cirsium japonicum DC. In this paper, the chemical fingerprint of Cirsiumjaponicum DC was established using raw materials from 15 origins in China. The chromatographic separations were obtained by a SHIM-PACK VP-ODS column （150 mm ~ 4.6 mm i.d., 5 ~tm） using gradient elution, and run time of 80 min. The peak of linarin was considered as the control peak. The experimental data were analyzed with the software of Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine （Version 2004A） and the quality control system of both the overall qualitative similari- ties and the overall quantitative similarities of traditional Chinese medicine chromatographic fingerprints.In many areas of China Cirsium setosum is used as Cirsiumjaponicum DC. Although the two herbs have similar appearance and many similar compounds, they are totally different medicinal material, and have different pharmacodynamic actions. The fingerprint spectrum can be a good tool to distinguish the two herbs and control the quality of Cirsium japonicum DC. In this paper, the chemical fingerprint of Cirsiumjaponicum DC was established using raw materials from 15 origins in China. The chromatographic separations were obtained by a SHIM-PACK VP-ODS column （150 mm ~ 4.6 mm i.d., 5 ~tm） using gradient elution, and run time of 80 min. The peak of linarin was considered as the control peak. The experimental data were analyzed with the software of Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine （Version 2004A） and the quality control system of both the overall qualitative similari- ties and the overall quantitative similarities of traditional Chinese medicine chromatographic fingerprints.

关 键 词：Cirsium japonicum DC FINGERPRINT High performance liquidchromatography-photodio dearray detec-tor （HPLC-PDA） LINARIN 

分 类 号：R284[医药卫生—中药学] O657.72[医药卫生—中医学]