玻璃体内持续释放肝素防治牵引性视网膜脱离的实验研究  

Biodegradable microspheres containing heparin in the treatment of proliferative vitreoretinopathy

作　　者：刘瑜玲[1] 陈改清[2] 张强[3] 徐亮[4] 张士元[4] 何蕾 

机构地区：[1]北京医科大学第三医院眼科,100083 [2]首都医科大学化学教研室 [3]北京医科大学药学院 [4]北京市眼科研究所

出　　处：《眼科研究》2000年第4期305-307,共3页Chinese Ophthalmic Research

基　　金：北京市教委科技发展基金

摘　　要：目的研究肝素聚乳酸微球（ＨＥＰ－ＭＳ）对兔牵引性视网膜脱离（ＴＲＤ）的防治效果。方法溶媒挥发法制备ＨＥＰ－ＭＳ，扫描电镜观察其形态，比色法测定其体外释放；在兔玻璃体内注入成纤维细胞后，分别注入空白微球，不注入／注入载药微球，观察 ＴＲＤ发生情况。结果微球球形较好，平均粒径 ６８． ６μｍ。对照及载药微球组在 ４周末时 ＴＲＤ的发生率分别为８０％和５０％，两者有显著性差异（Ｐ＜０．０５）。结论玻璃体内一次性注入ＨＥＰ－ＭＳ能够有效地减少ＴＲＤ的发生。Objective To investigate the possibility of biodegradable heparin polylactic acid microspheres (HEP-MS) for the treatment of proliferative vitreoretinopathy (PVR) in an experimental rabbit model. Methods Biodegradable microspheres containing heparin was prepared by solvent evaporation method. Morphology of HEP-MS was examined by the scanning electron microscope. The loading copacity of heparin in HEP-MS was analyzed by a fluorospectro - photometer, an d th e in vitro release of heparin from the HEP-MS was also investigated. Twenty pigmented rabbits (40 eyes) were divided into two groups. The blank microspheres (10 eyes) and phosphate buffer solution (PBS) (10 eyes) were injected intravitreally as the control. The HEP-MS containing 1. 42 mg/ml(20 eyes) of HEP were injected into the other group. 7 d after second gas-compression vitrectomy,　2×10 fibroblastes and HEP-MS (or control) were injected into all eyes sequentially. The morbidity of tractional retinal detachment (TRD) were observed by ophthalmoscope for 4 weeks. Results The HEP-MS was quite round, and the mean diameter of HEP-MS was 68.6μm. The loading capacity of heparin in HEP-MS was 1. 42%. 4 weeks later, the morbidity of TRD in control, 1. 42mg/ml HEP-MS group was 80%, and 50% respectively. Significant statistical difference was found between the control group and the HEP-MS group(P<0. 05 ). Conclusion The microspheres containing 1. 42 mg/ml of HEP can inhibit the occurrence of TRD effectively.

关 键 词：生物降解性微球 聚乳酸 肝素 牵引性视网膜脱离 

分 类 号：R774[医药卫生—眼科]