克伦特罗和马布特罗在替考拉宁手性柱上的对映体分离  

Enantioseparation of Clenbuterol and Mabuterol on the Teicoplanin Chiral Stationary Phase

作　　者：韩亚琼[1] 余小燕[1] 谷晓娟[1] 沈报春[1] 

机构地区：[1]昆明医科大学药学院暨云南省天然药物药理重点实验室,云南昆明650500

出　　处：《昆明医科大学学报》2013年第6期12-16,共5页Journal of Kunming Medical University

基　　金：国家自然科学基金资助项目(81102408);昆明医科大学本科试验药学示范中心资助项目(2012)

摘　　要：目的研究自制的替考拉宁手性色谱柱上对克伦特罗和马布特罗进行对映体分离,探讨其手性识别机理.方法在流动相组成的甲醇/乙醇/冰醋酸/三乙胺=50/50/0.05/0.05(V/V/V/V),流速1.0 mL/min,柱温25℃.应用了极性流动相中不同浓度的酸碱添加剂,不同浓度的乙醇,柱温和流速对克伦特罗和马布特罗对映体进行分离.结果在上述条件下,克伦特罗对映体和马布特罗对映体的选择因子分别达到1.37和1.51.结论自制的替考拉宁手性柱对克伦特罗和马布特罗的对映体具有一定的分离效果.Objective Using the polar mobile phase, the enantioseparation of clenbuterol and mabuterol on the self-made teicoplanin chairal stationary phase were studied. Methods The mobile phase consisted of methanol ： ethanol：acetic acid：triethylamine = 50：50：0.05：0.05 （V/V/V） with a flow rate of 1.0 mlJmin and column temperature of 25 ~C. The influences of the proportion of acetic acid and triethyamine, ethanol concentration, column temperature and flow rate on the enantioseparation were investigated. The chiral recognition mechanism was also discussed. Results Under the optimal conditions, the separation factor for clenbuterol and mabuterol was 1.37 and 1.51, respectively. Conclusion The result showed that the isomers of clenbuterol and mabuterol could be separated on the self-made teicoplanin chairal stationary phase in the polar mobile phase.

关 键 词：对映体分离 克伦特罗 马布特罗 手性识别机理 

分 类 号：TQ460.72[医药卫生—药物分析学]