甘蔗糖蜜酒精废液产腐殖酸的菌株筛选与鉴定  

Isolation and Identification of Microorganism Producing Humic Acid with Sugarcane Molasses Alcohol Wastewater

作　　者：李楠[1,2] 邓智年[1,3] 奉灵波[2] 周瑞芳[2] 魏源文[1,3] 曹辉庆[1,3] 李杨瑞[1,3] 

机构地区：[1]广西农业科学院,广西南宁530007 [2]广西大学生命科学与技术学院,广西南宁530004 [3]广西作物遗传改良生物技术重点开放实验室,广西南宁530007

出　　处：《广西师范大学学报（自然科学版）》2013年第2期87-92,共6页Journal of Guangxi Normal University:Natural Science Edition

基　　金：国家自然科学基金资助项目(31171504);第46期中国博士后科学资金资助项目(20090460841);广西农科院博士后资金资助项目(桂农科博2009012)

摘　　要：以甘蔗糖蜜酒精废液作为筛选培养基,从土壤中筛选出一株能利用酒精废液为原料发酵生产腐殖酸的细菌AS019,通过形态特征、培养及生理特征及生理生化特征研究,结合16S rDNA序列分析比对,进行菌株鉴定,并利用红外光谱测试分析发酵产物腐殖酸的结构。实验结果表明:初步鉴定菌株AS019为芽孢杆菌属的巨大芽孢杆菌Bacillus megaterium,发酵前后腐殖酸含量分别为6.60和25.33g/L,发酵产品腐殖酸含有多种活性官能团。可见,甘蔗糖蜜酒精废液可以作为微生物培养基发酵生产出高附加值腐殖酸,解决了糖厂酒精废液处理问题,对甘蔗酒精废液的开发利用、环境保护和蔗糖业循环经济发展等具有重要意义。Microbial strain AS019 suited to grow in vinasse and be able to produce rich HA,was screened from soil.Combined with the colony morphology,physiological and biochemical characteristics and 16S rDNA ITS sequence,AS109 was preliminarily identified as Bacillus megaterium.After fermented by strain AS019,the content of humic acid in vinasse was increased from 6.60 g/L to 25.33 g/L.Humic acid structure determined by infrared spectra is rich in active functional groups,such as hydroxyl（OH）,carboxyl（-COOH）,carbonyl（-C=O）,amino and high aromaticity as well.The results indicated that vinasse could be used as microbiological medium to produce the high value-added humic acid.It was of great significance to the utilization of vinasse,environmental protection and circular economy development in the sugarcane industry.

关 键 词：酒精废液 腐殖酸 菌株分离鉴定 生理生化特征 红外光谱 

分 类 号：Q93-33[生物学—微生物学] TS249.3[轻工技术与工程—制糖工程]