肺癌组织端粒酶活性的定量研究及其临床意义  

A study on the telomerase activity in lung cancer and its clinical significance

作　　者：陈蔚蔚[1] 周宏远[1] 周清华[1] 熊小熊[1] 张洁[1] 

机构地区：[1]华西医科大学附属第一医院肿瘤中心,肿瘤研究所,成都610041

出　　处：《中国肺癌杂志》2000年第2期88-90,共3页Chinese Journal of Lung Cancer

基　　金：国家自然科学基金!(39570 392 )资助

摘　　要：目的　研究肺癌组织中端粒酶活性的表达及其临床意义。方法　采用定量放射端粒重复序列扩增法 (TRAP)检测 74例肺组织中 ( 4 5例为NSCLC ,15例SCLC ,结核 7例 ,炎性假瘤 7例 )端粒酶的活性。结果　NSCLC端粒酶阳性率为 73 .3 % ,且活性较弱 ( 172TPG) ,SCLC端粒酶的阳性率为 93 .3 % ,且活性较强 ( 2 88TPG)。结核与炎性假瘤端粒酶活性全为阴性。SCLC端粒酶活性显著高于NSCLC(P <0 .0 5 ) ,且酶的活性水平与肺癌组织分化程度、肿瘤大小、淋巴结转移及临床分期有密切关系 (P <0 .0 5 )。结论　端粒酶可能是判断肺癌预后不良的指标之一。Objective To investigate the telomerase activity in human lung cancer and its clinical significance. Methods Sixty human lung cancer specimens (NSCLC 45, SCLC 15), seven tuberculosis specimens and seven inflammatory pesudotumor were examined for telomerase activity by means of TRAP (telomeric repeat amplification protocol) based on polymerase chain reaction. Results In NSCLC, telomerase positivity was 73.3%(33/45) with weak to moderate activity (172?TPG), whereas 14 of 15 SCLC (93.3%) were positive with strong activity (288?TPG), and all tuberculosis and inflammatory pseudotumor were telomerase negativity. The telomerase activity was significantly higher in SCLC than that in NSCLC (P<0.05), and was closely associated with the differentiation, size of the cancer, lymph node metastasis, and TNM staging (P<0.05). Conclusion The results suggest that telomerase may be regarded as one of the prognostic factor in lung cancer.

关 键 词：肺肿瘤 端粒酶 TARP-PCR 活性 

分 类 号：R734.2[医药卫生—肿瘤]