人肺癌端粒酶活性的研究  

Detection of telomerase activity in human lung cancer

作　　者：崔旭[1] 张京岚[2] 周奕[2] 胡尚基[2] 张京立[1] 高明哲[2] 张洁[1] 

机构地区：[1]解放军总医院老年医学研究所,北京100853 [2]首都医科大学附属北京安贞医院

出　　处：《中国肺癌杂志》2000年第2期104-106,共3页Chinese Journal of Lung Cancer

摘　　要：目的　探讨端粒酶活性表达与肺癌发生发展的关系。方法　应用PCR TRAP 银染法检测5 0例肺癌组织及细胞和 2 0例相应癌旁组织、5例肺结核瘤组织及细胞的端粒酶活性。结果　肺癌组 :3 6例手术切除肺癌组织中端粒酶阳性率为 91.7% ,7例纤维支气管镜活检肺癌组织和 7例癌性胸水细胞中端粒酶阳性例数分别为 6例和 5例 ;总检出率为 88% ( 4 4/5 0 )。对照组 :癌旁组织中端粒酶阳性率为10 % ( 2 /2 0 ) ,肺结核瘤组织及细胞的端粒酶表达均呈阴性。将纤支镜活检组织分别进行端粒酶表达和病理检查 ,发现端粒酶活性检出率 ( 6/7)明显高于病理诊断阳性率 ( 3 /7)。结论　端粒酶可作为肺癌诊断的指标之一 ,检测其在纤维支气管镜活检组织及胸水细胞中的表达可提高肺癌早期诊断率。Objective To investigate the role of telomerase activity in the oncogenesis and progression of human lung cancer. Methods A total of 50 malignant neoplasm's specimens from patient with lung cancer including 36 surgically resected tissues, 7 fiberobronchoscopically collected tissues or cells, 7 pleural effusion cells and 20 tumor adjacent tissues and 5 tuberculous tissues or cells were examined for telomerase activity by PCR based TRAP(telomeric repeat amplification protocol) assay. Results In the lung cancer group, the positive rate of telomerase activity in surgically resected lung cancer tissues was 91.7%(33 out of 36 samples), and 85.7% (6 out of 7 samples) and 71.4% (5 out of 7 samples) in fiberobronchoscopically collected tissues or cells and pleural effusion cells, respectively. However, that in the tumor adjacent tissues was 10% (2 out of 20 specimens) and no telomerase activity was observed in 5 tuberculous tissues or cells. Positive rate in telomerase activity (85.7%) was significantly higher than that in pathologic assay ( 42.9% ) in the same 7 samples collected through fiberobronchoscope. Conclusion The results suggest that detection of telomerase activity of fiberobronchoscopically collected tissues or cells and pleural effusion cells would be a helpful examination in the diagnosis of patients with lung cancer.

关 键 词：肺肿瘤 端粒酶 活性 PCR-TRAP-银染法 

分 类 号：R734.2[医药卫生—肿瘤]