人类鼻咽癌端粒酶活性的研究及其临床意义  

The study on the role of telomerase activity in human nasopharyngeal carcinoma

作　　者：彭宏[1] 王希军[1] 杨光彩[2] 江肃萍 赵彤[3] 

机构地区：[1]广州第一军医大学南方医院耳鼻咽喉科,510515 [2]第一军医大学生物化学教研室 [3]第一军医大学病理学教研室

出　　处：《中华耳鼻咽喉科杂志》2000年第4期289-291,共3页Chinese Journal of Otorhinolaryngology

摘　　要：目的　探讨端粒酶在鼻咽癌中的表达及其临床意义。方法　采用TRAP 银染法对 42例鼻咽部低分化鳞状细胞癌、10例鼻咽部正常粘膜和 8例鼻咽纤维血管瘤进行端粒酶活性检测及血清VCA/IgA检测 ,并分析其与临床病理的关系。结果　鼻咽部正常粘膜和鼻咽癌端粒酶阳性率分别为 2 0 %和 88.1% ,8例鼻咽纤维血管瘤均未检测到端粒酶活性。鼻咽癌伴颈部淋巴结转移者端粒酶阳性率高于无颈部淋巴结转移者 (P =0 .0 35 ) ,临床晚期 (III、IV)端粒酶阳性率高于临床早期 (I、II) (P=0 .0 48) ,而端粒酶与鼻咽癌患者的年龄、性别及远处转移均无明显相关性 (P >0 .0 5 )。鼻咽癌患者血清EB病毒VCA/IgA阳性率明显高于鼻咽粘膜正常者 (P <0 .0 0 1)。结论　端粒酶活性表达在鼻咽癌发病机制中起着重要作用。端粒酶活性结合血清EB病毒抗体的检测有利于鼻咽癌的早期诊断。Objective To elucidate the role of telomerase activity in nasopharyngeal carcinoma (NPC).Methods Telomerase activity was detected using the telomere repeat amplification protocol(TRAP) combined with silver staining in 42 low-differentiated squamous carcinoma, 10 normal nasopharyngeal mucosae and 8 nasopharyngeal fibrohemangioma. Results Telomerase activity was detectable in 20% normal nasopharyngeal mucosae and 88.1% in NPC. The activity of telomerase was not detectable in 8 nasopharyngeal fibrohemangioma tissues. Telomerase activity was present more frequently in NPC biopsies with positive lymph node metastasis than in those without lymph node metastasis ( P =0.035). Furthermore, telomerase activity was observed more frequently in advanced NPC than in early stage ( P =0.048). However, there was no evidence to show the relationship between telomerase activity and other clinical features, such as age, gender and distant metastasis ( P >0.05). Serum EBV marker(VCA-IgA) was observed more frequently in NPC than in normal nasopharyngeal mucosae patients( P <0.001). Conclusion Telomerase activity may play a critical role in the tumorigenesis of NPC. Detection of telomerase activity combined with serological EBV markers may contribute to the screening and early diagnosis of NPC.

关 键 词：鼻咽肿瘤 端粒 端粒酶 淋巴转移 EB病毒 

分 类 号：R739.6[医药卫生—肿瘤]