杨树芽提取物清除DPPH自由基的作用  

DPPH radical scavenging effect of poplar bud extracts

作　　者：刘长龙[1] 雷慧[1] 赵其秀[1] 李海涛[1] 

机构地区：[1]南京中医药大学药学院,江苏南京210023

出　　处：《安徽医药》2013年第7期1091-1093,共3页Anhui Medical and Pharmaceutical Journal

基　　金：江苏省"六大人才高峰"项目资助(No YY-111)

摘　　要：目的对杨树芽进行初步提取分离,测定其总黄酮含量和抗氧化活性。方法通过超声辅助提取杨树芽中有效成分,以抗坏血酸、生育酚和芦丁为对照品,采用DPPH法测定其自由基清除作用。结果超声辅助提取的杨树芽提取物中总黄酮含量为14.4%,浓度为0.05 g.L-1时,清除自由基能力大于芦丁和生育酚,浓度提高后略低于生育酚,高于芦丁,且清除能力随浓度提高而提高。结论杨树芽提取物中总黄酮含量较高,并有较强的抗氧化活性。Objective To make preliminary extraction and separation of poplar buds,and to determination its total flavonoids and antioxidant activity.Methods By ultrasound we extracted the active ingredients in the poplar buds.Ascorbic acid,tocopherol and rutin as reference,we measured free radical scavenging effect by DPPH method.Results Ultrasonic assisted extraction of poplar buds contained 14.4% of total flavonoids,whose radical scavenging capacity was higher than rutin and tocopherol when its concentration reached 0.05 g.L-1.With increased concentration the scavenging capacity was slightly lower than tocopherol but higher than rutin.And the capacity increased with the increase of concentration.Conclusion Poplar bud extracts had high flavonoid content and strong antioxidant activity.

关 键 词：杨树芽提取物 总黄酮 DPPH自由基 

分 类 号：R284[医药卫生—中药学]