福建省耐多药结核分枝杆菌利福平耐药rpoB基因突变特征  

Mutations of rpoB gene in multi-drug resistant Mycobacterium tuberculosis isolates from Fujian province

作　　者：梁庆福[1] 陈求扬[1,2] 林淑芳[1] 逄宇[3] 魏淑贞[1] 赵永[1] 

机构地区：[1]福建省疾病预防控制中心,福州350001 [2]福建省医科大学公共卫生学院教学基地,福州350008 [3]中国疾病预防控制中心,结核病预防控制中心,国家结核病参比实验室,北京102206

出　　处：《中国卫生检验杂志》2013年第7期1649-1651,共3页Chinese Journal of Health Laboratory Technology

基　　金：福建省自然科学基金课题资助项目(2010J01116)

摘　　要：目的:分析福建省耐多药结核分枝杆菌利福平耐药相关基因rpoB的突变特征,为福建省耐多药结核病快速诊断方法的建立提供科学依据。方法:对来源于福建省结核病耐药性监测30个监测点纳入的75株耐多药和10株全敏感结核分枝杆菌分离菌株,应用PCR扩增包含rpoB耐药决定区(RRDR)的基因片段,测序后以结核分枝杆菌H37Rv标准株DNA序列为参比序列,分析菌株的突变特征。结果:10株全敏感株未检测到突变。75株耐多药结核分枝杆菌检测到72株rpoB基因发生突变,突变率为96.0%(72/75),突变在RRDR区域内占94.7%(71/75)。共有15种突变类型,包括12种点突变和3种联合突变,涉及9个突变位点,8个突变位点位于RRDR区内,1个在RRDR外,为569位点。最常见位点突变依次是531、526和516,三者突变率之和达84.0%(63/75)。结论:福建省耐多药结核分枝杆菌RFP耐药性快速诊断方法的建立可基于531、526和516这3个位点的突变特征。Objective： To analyze the mutation characteristics of rpoB gene in multi - drug resistant （MDR） My- cobacterium tuberculosis isolates in Fujian province, and provide molecular evidence for fast diagnosis of multi - drug resistant tuberculosis. Methods： Seventy - five MDR M. tuberculosis isolates and 10 pan - susceptible isolates were selected from 30 survey sites, and the mutation patterns including RRDR in rpoB gene of these strains were detected by PCR and sequenced with H37Rv as control. Results ： No mutation was detected in 10 pan - susceptible isolates. In 75 MDR M. tuberculosis isolates, the mutation rate of rpoB gene was 96.0% （72/75）, mainly in RRDR region （94.7%, 71/75 ）. It was found 15 types of mutations, including 12 point mutations and 3 combination mutations, involving 9 sites in RRDR region and 1 site not（locus 569）. The most frequent mutation sites in MDR M. tuberculosis isolates from Fujian province were codon531 ,codon526 and codon516, with total mutation rate of 84.0% （ 63/75 ）. Conclusion： The rapid diagnosis method for RFP drug resistance of MDR M. tuberculosis could be based on the 3 loci mutation characteristics

关 键 词：结核分枝杆菌 耐多药 利福平 RPOB基因 突变 

分 类 号：R378.911[医药卫生—病原生物学]