人乳头瘤病毒感染及基因型别分析  

Infection and genotypes of human papillomavirus

作　　者：张正国[1] 贺庆伟[1] 

机构地区：[1]河南省中医院检验科,河南郑州450002

出　　处：《中国卫生检验杂志》2013年第7期1757-1758,共2页Chinese Journal of Health Laboratory Technology

摘　　要：目的:探讨人乳头瘤病毒基因分型检测在宫颈疾病诊疗中的分布特点及临床应用。方法 :采用PCR扩增法与膜杂交技术检测21种HPV基因型别并分析。结果 :在837例临床样本中,HPV基因分型亚型阳性400例,阳性率占47.79%,其中高危亚型274例,占32.74%,低危亚型56例,占6.69%;单一HPV感染为231例,占27.60%,双重感染共为110例,占13.15%;多重感染(低、高危两种以上)共为59例,占7.05%。结论:郑州地区人群中,存在较高的HPV感染率,高危型感染率较高。HPV基因分型检测对于宫颈癌筛查有一定的参考价值。Objective ： To investigate the distribution characteristics and clinical application of human papilloma virus genotyping in the diagnosis and treatment of cervical disease. Methods： PCR amplification and membrane hy- bridization technique were employed to detect and analyze 21 HPV genotypes. Results： In 837 clinical samples, HPV gene subtypes were found in 400 cases , occupying 47.79% , in which 274 cases were high - risk subtypes, accounting for 32.74% , 56 cases were low - risk subtypes, accounting for 6.69%. Meanwhile, there were total 231 single HPV infection cases, 110 double infection cases and 59 multi -infection cases, accounting for 27.60%, 13.15 %, 7.05 % respectively. Conclusion ： Higher prevalence and risk of the HPV infection was found in Zhengzhou. HPV genotyping test had a certain reference value in cervical cancer screening.

关 键 词：人乳头瘤病毒 膜杂交技术 基因分型 宫颈癌 

分 类 号：R737.33[医药卫生—肿瘤]