宫颈组织病变与检测HPV基因分型的意义  

The significance of HPV genotyping in cervical lesions

作　　者：张达衡[1] 陈红玲[1] 杨春媚[1] 陈瑞林[1] 

机构地区：[1]广东省茂名市人民医院检验科,525000

出　　处：《国际检验医学杂志》2013年第13期1664-1665,共2页International Journal of Laboratory Medicine

摘　　要：目的了解宫颈病变患者人乳头瘤病毒HPV感染率及基因型分布情况。探讨人乳头瘤病毒基因分型与宫颈组织病变的相关性,为临床宫颈癌的筛查、防治提供参考。方法收集在本院诊治2 000例疑似宫颈病变女性,应用基因芯片技术检测23种HPV基因型,统计HPV各基因型的感染率,分析HPV及基因型分布与子宫病变的关系。结果 2 000例宫颈疑似宫颈病变女性中,基因芯片检测出的HPV阳性标本662例,HPV感染率为33.1%(662/2 000)。354例宫颈不同程度病变组织中HPV阳性295例,其中LSIL,HSIL及宫颈癌样本中HPV的检出率分别为76.6%(157/205)、91.9%(125/136)、100%(13/13)。共检出12种HPV基因型,检出率最高的5种基因型分别为HPV16,18,58,31,52。宫颈病变级别越高,HPV多重感染率越高。结论 HPV亚型感染与宫颈组织不同程度病变密切相关,HPV基因分型检测及诊断在宫颈病变及宫颈癌筛查及防治过程中具有重要意义。Objective To investigate the infection rate of human papillomavirus（HPV） and distribution of HPV genotypes in cervical lesions,and discuss the correlation between HPV genotypes and cervical lesions to provide a reference for cervical cancer screening,prevention and treatment.Methods 2 000 cases of women with suspected cervical lesions were detected by 23 cases of HPV genotyping based on gene chip technology,and infection rates of HPV genotypes and the correlation between HPV genotypes and cervical lesions were analyzed.Results 662 cases were HPV positive in 2 000 cases of women with suspected cervical lesions,with the HPV infection rate of 33.1%（662/2 000）,and 295 cases were HPV positive in 354 cases of women with cervical lesions.For LSIL,HSIL and cervical cancer,HPV detection rates were 76.6%（157/205）,91.9%（125/136）,100%（13 / 13）,respectively.12 kinds of HPV genotypes were detected,and the top five genotypes of the highest detection rate were HPV16,18,58,31,52.The higher the level of cervical lesions,HPV multiple infection was higher.Conclusion HPV infection and cervical lesions is closely related,HPV genotyping is important in the cervical cancer screening and prevention.

关 键 词：宫颈疾病 乳头状瘤病毒科 基因型 

分 类 号：R737.33[医药卫生—肿瘤]