氨基酸维生素对力竭游泳大鼠腓肠肌超微结构和相关酶的影响  

Influence of amino acids and vitamins on the ultrastructure of calf skeletal muscle and relevant enzymes in exhausted swiming rats

作　　者：贾文斌[1] 孙金山[2] 徐纪平[2] 惠华强[2] 黄文[2] 

机构地区：[1]济南军区青岛第一疗养院,山东青岛266071 [2]第二军医大学长海医院,上海200433

出　　处：《实用医药杂志》2013年第7期616-618,共3页Practical Journal of Medicine & Pharmacy

基　　金：军队"十一五"医药卫生科研基金重点课题(08Z022);广东省深圳市科技局2009年重点课题

摘　　要：目的观察氨基酸维生素制剂对力竭游泳大鼠腓肠肌超微结构和相关酶影响。方法 36只雄性SD大鼠经适应性游泳后,随机分为空白对照组、复方氨基酸胶囊组(胶囊组)和氨基酸果糖粉固体饮料组(冲剂组),采用无负重游泳方式建立大鼠力竭游泳模型。分别给予正常饮用水、复方氨基酸胶囊和氨基酸果糖饮料配方饮水21 d后,检测3组大鼠无负重游泳力竭时间,电镜观察检测大鼠腓肠肌超微结构,ELISA法检测大鼠腓肠肌组织中肌酸激酶(CK)、乳酸脱氢酶(LDH)、一氧化氮(NO)和一氧化氮合酶(NOS)水平。结果胶囊组和冲剂组游泳力竭时间显著长于对照组(P<0.05)。胶囊组和冲剂组腓肠肌细胞胞质内线粒体和肌丝结构基本完整,对照组腓肠肌细胞则出现肿胀变性,线粒体嵴松散、弯曲、溶解、断裂和空泡化。胶囊组和冲剂组大鼠腓肠肌组织中CK和LDH均明显低于对照组(P<0.05),NO和NOS则高于对照组(P<0.05)。结论补充复方氨基酸维生素制剂可通过减轻腓肠肌损伤,调节肌肉损伤相关酶来提高大鼠抗疲劳能力。Objective To observe the effects of amino acids and vitamines on the ultrastrueture of calf skeletal muscle and related enzymes in exhausted swiming rats.＇ Methods The 36 SD male rats were randomly divided into 3 groups： blank group, capsule group and power preparation group. All rats were established as exhaust fatigue models by swimming. Three groups were treated with normal drinking water, amino acids capsule and amino acid-fructose beverage respectively for 21 days. After the last exhaused swimming, the uhrastructure of calf skeletal muscle were observed by electron microscope. The contents of creatinkinase（CK）, lactate dehydrogenase （LDH）, nitrogen monoxidum （NO） and nitrogen monoxidum synthase （NOS） in skeletal muscle were detected by ELISA. Results The exhausted swiming time in capsule and the granules group was significant longer than that of the control group （P〈0.05）. The uhrastructure of chondriosome and myofilament from skeletal muscle in capsule and the granules groups were of fundamental integrity. There were obviously engorgement and apomorphosis in control group, whose ehondriosome showing difference degree of damage accompanied with loosing, gryphosis, solution, fragmentation and even vaeulization in membrance and erista. Compared with the control group, the contents of CK and LDH in skeletal muscle of the capsule and powder groups were significantly lower（P〈0.05）, as well as the contents of NO and NOS were significantly higher （P〈0.05）. Conclusion Supplement of amino acids and vitamines can increase resistance to fatigue by reducing uhrastructural injury of skeletal muscle and adjusting the related enzymes.

关 键 词：氨基酸 维生素 力竭疲劳 超微结构 

分 类 号：R393[医药卫生—基础医学]