一株耐铅细菌的分离鉴定及其吸附特性研究  

Isolation,identification and Pb(Ⅱ) biosorption characterization of a lead-resistant strain

作　　者：金羽[1,2] 曲娟娟[1] 李影[1] 顾海东[1] 闫立龙[1] 孙兴滨[3] 

机构地区：[1]东北农业大学资源与环境学院,哈尔滨150030 [2]哈尔滨工业大学城市水资源与水环境国家重点实验室,哈尔滨150090 [3]东北林业大学林学院,哈尔滨150040

出　　处：《环境科学学报》2013年第8期2248-2255,共8页Acta Scientiae Circumstantiae

基　　金：环保公益性行业科研专项资助项目(No.2010467038)~~

摘　　要：从某铅矿区土壤中筛选出一株耐铅细菌,对其进行初步鉴定,并对Pb2+吸附特性和机理进行研究.16SrDNA序列相似性分析表明,该菌属于节杆菌属,将其命名为Arthrobacter scleromaeLY-1.菌株最大耐Pb2+浓度为500mg.L-1,此外,菌株对Zn2+、Cu2+、Ni2+、Co2+等重金属也有一定的耐受性.在初始Pb2+浓度为100mg.L-1、投菌量(鲜重)为10g.L-1、pH为6.0、温度为30℃、吸附时间为25min时,吸附Pb2+效果较好,此时吸附率为99.61%,吸附容量为9.96mg.g-1,吸附等温方程符合Langmuir模型.透射电镜观察和红外光谱分析显示,菌株LY-1对Pb2+的吸附主要是细胞表面的吸附,菌体细胞表面的多种活性基团与Pb2+发生络合作用.A lead resistant bacterium was isolated from heavy metal-contaminated soil in a lead-zinc mining area.The bacterium was preliminarily identified and its Pb2＋ removal characteristics were analyzed.The 16S rDNA sequence analysis revealed that this bacterium belongs to Arthrobacter sp.and was named as A.scleromae LY-1.Strain A.scleromae LY-1 possesses the capability of Pb2＋ resistance up to 500 mg · L-1 in aqueous solution.In addition,it also has certain resistance to other heavy metals such as Zn2＋,Cu2＋,Ni2＋ and Co2＋.Optimum conditions for maximum biosportion were found at pH of 6.0 with inoculation quantity of 10 g · L-1（fresh weight）,initial Pb2＋ concentration of 100 mg · L-1,and contact time of 25 min at 30 ℃.Under such conditions,the adsorption rate and capacity reached 99.61% and 9.96 mg · g-1,respectively.Efficient performance of the biosorbent is in accordance with Langmuir isotherm model.Transmission electron microscope（TEM） coupled with Fourier Transform InfraRed（FT-IR） results revealed that the biosorption process mainly happens on the surface of LY-1 cell and many active groups on the extracellular matrix can chelate the lead ions.

关 键 词：铅 吸附 节杆菌 

分 类 号：X172[环境科学与工程—环境科学]