鸡源大肠杆菌OMP和OmpT基因与血清型相关性  

Correlation of poultry pathogenic E.coli OMP and OmpT gene with serotype

作　　者：贾青辉[1] 张艳英[1] 史秋梅[1] 房海[1] 陈翠珍[1] 张香斋[1] 李佩国[1] 

机构地区：[1]河北科技师范学院河北预防兽医学重点实验室,河北昌黎066600

出　　处：《中国兽医学报》2013年第8期1217-1222,共6页Chinese Journal of Veterinary Science

基　　金：河北省科技支撑计划资助项目(08220401D;10960408D)

摘　　要：为了解禽致病性大肠杆菌分离株0MP、OmpT基因与血清型相关性，对6种血清型（O78、O38、O9、O91、O11、O157）的7株鸡源大肠杆菌菌株进行了外膜蛋白（OMP）和外膜蛋白酶T（OmpT）基因的研究。用N-十二烷酰肌氨酸法提取其外膜蛋白，经SDS-PAGE电泳后考马斯亮蓝染色，对鸡源大肠杆菌菌株进行了外膜蛋白分型；用PCR和生物信息学的方法对OmpT基因进行检测与分析。7株大肠杆菌共有3个OMP型，其中，分离株5，32，9主要由相对分子质量接近的2条带组成，为OMP-1型；分离株11主要由相对分子质量接近的3条带组成，为OMP-2型；分离株14，7主要由相对分子质量接近的1条带组成，为0MP-3型。这表明同一血清型的菌株可能属于不同的OMP型，而血清型不同的分离株之间却可具有相同的0MP型。PCR检测结果显示7株鸡源大肠杆菌均携带OmpT基因，与GenBank登录的序列比较发现其同源性为91．9％～100．0％，不同血清型菌株间的同源性为91．9％～98．0％。该试验证实了同一血清型分离株之间可发生遗传分化，而不同血清型分离株之间也可具有不同程度的遗传相关性；不同血清型分离株间的OmpT基因的同源性存在一定的差异。In order to understand the correlation of poultry pathogenic E. coli separate strains OMP and OmpT with serotype,the genes of outer membrane protein （OMP） and outer membrane pro- tease T （OmpT） were studied for 7 strains poultry pathogenic E. coli with six serotypes （078, O38,O9, O91, O11, O157）. The outer membrane protein was extracted with N-dodecane sarco-sine, after SDS-PAGE electrophoresis, coomassie blue staining, genotyped poultry pathogenic E. coli outer membrane protein. The OmpT gene were detected and analysised by PCR and bioinfor-matics. Three OMP types were found from seven strain E. coli, the isolates strain of 5,32,9 were mainly composed by the two bands with the similar molecular weight,as OMP-1 type;the isolates strain of 11 mainly composed by the three bands with similar molecular weight,as OMP-2 type; the isolates strain of 14,7 mainly composed by the one band with the similar molecular weight,as OMP-3 type. This indicated that the strains with the same serotype may belong to different OMP type,the strains with the different serotypes may have the same OMP-type. Seven chicken E. coli strains carrying the OmpT gene were showed by PCR and sequence were comparison with Gen-bank accession,and found that the homology were between 91.9%0-100.0%, the homology of dif-ferent serotypes were 91.9%-98.0%. The homology of different serotypes of the isolates OmpT gene had some differences. The test confirmed that the same serotype strains may produced genetic differentiation,and there also was some genetic correlation between different serotype isolates;the homology of OmpT gene have some differences between different serotypes isolates.

关 键 词：鸡源大肠杆菌 外膜蛋白 血清型 OMPT SDS-PAGE 

分 类 号：S852.612[农业科学—基础兽医学]