兔实验性急性高眼压模型视网膜谷氨酸的变化  

Glutamate in experimental acute elevated intraocular pressure models of rabbits

作　　者：王平宝[1] 蒋幼芹[2] 黄佩刚[1] 陈主初[3] 

机构地区：[1]湖南医科大学湘雅医院眼科,长沙410008 [2]湖南医科大学附属第二医院眼科 [3]湖南医科大学肿瘤研究所

出　　处：《中华眼科杂志》2000年第5期378-380,共3页Chinese Journal of Ophthalmology

摘　　要：目的　研究兔实验性急性高眼压状态下视网膜谷氨酸含量的变化 ,探讨谷氨酸在青光眼视神经损害中的作用。方法　应用邻苯二甲醛衍生法、高效液相色谱仪测定视网膜谷氨酸的含量。将 18只大白兔分成 3组 ,其中 2个实验组 ,1个正常对照组 ,每组均 6只兔 (6只眼 )。实验 1组 :每只兔随机选取 1只眼行生理盐水前房内高压灌注 ,6 0mmHg(1mmHg=0 .133kPa)持续 4h。另 1只眼生理盐水前房内灌注 ,方法同前 ,但压力为 2 0mmHg作自身对照。实验 2组 :每只兔随机选 1只眼用 30mmHg压力前房生理盐水灌注 ,另 1只眼 2 0mmHg灌注作自身对照 ,持续 4h。正常对照组不做前房灌注 ,每只兔任选 1只眼。以上各组均在摘除眼球后做视网膜谷氨酸测定。结果　实验 1、2组兔的高眼压眼 (6 0mmHg,30mmHg)均较对侧眼 (2 0mmHg)谷氨酸水平明显增高 (P <0 .0 5 )。结论　急性高眼压可导致视网膜谷氨酸水平增高 。Objective To measure the concentration of glutamate (Glu) in the retina of eyes with experimentally elevated intraocular pressure (IOP) Methods High performance liquid chromatography (HPLC) was utilized to measure the Glu in the retina 18 rabbits were divided into three groups, each group including 6 rabbits In one eye of each rabbit in group 1, the IOP was elevated to 60 mm Hg (1 mm Hg=0 133 kPa) by the anterior chamber perfusion with normal saline maintained for 4 hours, while the IOP of the other eye was maintained at 20 mm Hg by the same method In group 2, the same experimental method was carried out, but the elevated IOP was maintained at 30 mm Hg Group 3 was a normal control group without perfusion Results High IOP eyes (60 mm Hg and 30 mm Hg) had high glutamate concentration in the retinal tissue than that in low IOP eyes (20 mm Hg) (P<0 05) Conclusion Elevated IOP induces higher content of glutamate in the retina

关 键 词：谷氨酸 视网膜 青光眼 急性高眼压 兔 

分 类 号：R-332[医药卫生]