线粒体DNA与人细胞癌变的关系——癌细胞核基因组mtDNA探针FISH分析  

Relationship between mitochondrial DNA(mtDNA) and carcinogenesis of human cells——Fluorescence in situ hybridization analysis in the nuclear genome of carcinoma cells with mitochondrial DNA probes

作　　者：胡义德[1] 钱桂生[1] 李懿[2] 刘素刚[1] 刘卫东[1] 赵自强[1] 曹世龙[3] 

机构地区：[1]第三军医大学附属新桥医院全军呼吸内科研究所,重庆400037 [2]第三军医大学附属新桥医院,临床管理处,重庆400037 [3]复旦大学医学院附属肿瘤医院,上海200032

出　　处：《中国癌症杂志》2000年第4期289-291,共3页China Oncology

基　　金：国家自然科学基金! ( 3 95 0 0 173 )

摘　　要：探讨mtDNA与细胞癌变的关系。方法 :采用PCR方法制备地高辛标记的 3条人mtDNA探针 ,对 6株人癌细胞系和 1例原代培养人皮肤成纤维细胞的染色体或间期细胞核进行荧光原位杂交分析。结果 :3条mtDNA探针在 6个癌细胞系的部分染色体或间期核中均存在阳性杂交信号 ,而在成纤维细胞核中未出现阳性杂交信号。结论 :表明癌细胞核基因组中存在mtDNA的同源序列 。Purpose:To explore the relationship between mitochondrial DNA(mtDNA) and carcinogenesis of human cells. Methods:Three kinds of non radioactive DIG labelled mtDNA probes were prepared by polymerase chain reaction (PCR). The chromosomes in metaphase or interphase nuclei of six human carcinoma cell lines and fibroblasts were analyzed by fluorescence in situ hybridization (FISH). Results:Hybridization signals were partly observed on the chromosomes in metaphase or interphase nuclei of six human carcinoma cell lines but not in fibroblasts. Conclusions:The mtDNA homology sequence exists in the nuclear genome of carcinoma cells and it might exert important effects on the carcinogenic process.

关 键 词：癌细胞系 线粒体DNA探针 荧光原位杂交 

分 类 号：R730.2[医药卫生—肿瘤]