大连地区不同年龄段女性人乳头瘤病毒感染及基因亚型分布调查  

Research on HPV infection and its genotypes distribution in female at different ages in Dalian area

作　　者：杨真[1] 侯剑[1] 李小欢[1] 袁小林[1] 

机构地区：[1]大连大学附属中山医院中心实验室,辽宁大连116001

出　　处：《现代预防医学》2013年第16期3024-3027,共4页Modern Preventive Medicine

摘　　要：目的探讨大连地区不同年龄段女性人乳头瘤病毒(HPV)的感染及基因亚型分布情况,为宫颈癌的筛查及防治提供理论依据。方法采用PCR体外扩增和DNA反向点杂交相结合的DNA芯片技术,对大连地区1341例成年女性HPV的感染情况进行定性检测并加以分型,按不同年龄分组,分别统计HPV感染阳性率、多重感染、基因型别及亚型分布情况。结果 1341例女性标本中,共检出HPV阳性标本280例,阳性率为20.88%;单一感染217例(占77.50%),双重感染41例(占14.64%),三重感染17例(占6.07%),四重感染5例(占1.79%);单纯低危型感染56例(占20.00%),单纯高危型感染196例(占70.00%),低危型与高危型混合感染28例(占10.00%);共检出20种基因型,包括低危型HPV6、11、42、43和高危型HPV16、18、31、33、35、45、51、52、53、56、58、59、66、68、73、83,低危型以HPV43感染为主,高危型以HPV16感染为主。结论对不同年龄段女性开展HPV分型检测对于HPV感染和宫颈癌的诊断、治疗具有重要意义。OBJECTIVE To explore the prevalence of human papillomavirus （HPV） infection and its genotypes distribution in female at different ages in Dalian area and to provide theoretical basis for screening and preventing cervical cancer. METHODS The infectious status of HPV in 1341 females in Dalian area was examined with gene chip technique and various genotypes of HPV were compared and analyzed. The positive rate, multiple infection, gene type and genotypes distribution were calculated respectively at different ages. RESULTS 280 of 1 341 cases were positive for HPV （20.88%）. Single, dou- ble, triplex and quadruplex genotypes of HPV accounted for 77.50% （217/280）, 14.64% （41/280）, 6.07% （17/280） and 1.79% （5/280）, respectively. Low-risk genotypes, high-risk genotypes and low-risk combined with high-risk genotypes accounted for 20.00% （56/280）, 70.00% （196/280） and 10.00% （28/280）, respectively. Twenty genotypes were detect- ed, including low-risk genotypes （HPV-6, 11, 42, 43） and high-risk genotypes （HPV-16, 18, 31, 33, 35, 45, 51, 52, 53, 56, 58, 59, 66, 68, 73, 83）, and the predominant genotypes were HPV43 and HPV16 respectively in low-risk and high-risk genotypes. CONCLUSION The determination of HPV genotypes was important for early discovery, diagnosis and treatment of cervical cancer.

关 键 词：人乳头瘤病毒 基因分型 年龄 宫颈癌 DNA芯片技术 

分 类 号：R730.261.6[医药卫生—肿瘤]