中国南海总合草苔虫抗癌活性成分研究III Bryostatin10,11,18的分离鉴定及其体外抗肿瘤活性  

STUDIES ON THE ANTINEOPLASTIC CONSTITUENTS FROM MARINE BRYOZOAN BUGULA NERITINA INHABITING SOUTH CHINA SEA (III):Isolation and Structural Elucidation of Bryostatin 10, 11 and 18

作　　者：林厚文[1] 易杨华 姚新生[3] 吴厚铭 

机构地区：[1]第二军医大学长征医院药材科,上海200003 [2]第二军医大学药学院天然药物化学教研室,上海200433 [3]沈阳药科大学,沈阳110015 [4]上海有机化学研究所,上海200031

出　　处：《中国海洋药物》2000年第4期1-5,共5页Chinese Journal of Marine Drugs

基　　金：国家自然科学基金!资助项目 (编号 2 96 72 0 5 0 )

摘　　要：对采自广西北海的总合草苔虫进行抗癌活性成分的追踪分离 ,经溶媒提取和多种层析方法 (SephadexL H- 2 0 ,ODS,HPL C等 )分离 ,得到 6个活性单体。利用电喷雾离子质谱 (ESI- MS)和高分辨核磁共振 (6 0 0MHz)二维谱技术 (DQF- COSY,HMQC,TOCSY,ROESY等 ) ,分别将其鉴定为 :bryostatin 4 ,bryostatin 5,bryostatin 6 ,bryostatin 10 ,bryostatin 11,bryostatin 18。其中 ,bryostatin 10 ,bryostatin 11,bryostatin 18是首次从中国产总合草苔虫中获得 ,并在体外表现出显著的抗癌活性。Six active compounds were isolated from the marine bryozoan Bugula neritina, inhabiting Nanwan Bay in South China Sea, by bioassay guided isolation method with a combination of extraction and partitionation with suitable solvent as well as multiple column chromatographies (Sephadex LH 20, ODS and preparative HPLC). Their structure were elucidated as known bryostatins:bryostatin 4,5,6,10,11 and 18 by intensive analysis of the data of high resolution 2D NMR (600 MHz, DQF COSY, TOCSY, HMQC and ROESY) and ESI MS. Among them, bryostatin 10, 11 and 18 were for the first time obtained from this bryozoan in South China Sea and showed significant antineoplastic activities in vitro.

关 键 词：总合草苔虫 抗癌活性 草苔虫内酯 ODS HPLC 

分 类 号：R282.74[医药卫生—中药学]