纤维素酶辅助提取沉香挥发油的工艺研究  

Cellulase-assisted extraction of essential oil from eaglewood

作　　者：弓宝[1] 杨云[1] 黄立标[1] 冯锦东[1] 

机构地区：[1]中国医学科学院北京协和医学院药用植物研究所海南分所海南省南药资源保护与开发重点实验室,万宁571533

出　　处：《中国新药杂志》2013年第15期1833-1835,1839,共4页Chinese Journal of New Drugs

基　　金：海南省中药现代化专项项目(2011ZY006)

摘　　要：目的:考查纤维素酶对沉香挥发油提取率的影响。方法:对沉香木粉碎的粒径、纤维素酶用量、酶解时间分别进行单因素观察实验,运用正交试验筛选出纤维素酶辅助提取沉香挥发油的最佳工艺条件。结果:沉香木粉碎过40目筛,纤维素酶用40 FPIU.(g沉香)-1、酶解时间2 h,该工艺条件下沉香挥发油提取得率可达到1.621‰,与未添加纤维素酶提取工艺相比,沉香挥发油提取得率增加了257%。结论:纤维素酶辅助提取沉香挥发油具有良好的应用前景。Objective ： To study the influence of cellulase on extracting essential oil from eaglewood. Meth- ods：Single factor experiments were conducted on particle size, extraction time and cellulase dose,then orthogonal experiments were carried out to find the optimum operating conditions. Results：The optimum conditions were as follows ： particle size 0. 425 mm, cellulase dose 40 FPIU （ Filter Paper International Units） · （ g, eaglewood） -1, and extraction time 2 h. Under the optimal conditions the essential oil yield reached 1. 621%o,increasing by 257% com- pared with the extraction without cellulase assistant. Conclusion：Cellulase-assisted extraction of essential oil from eaglewood has a good application prospect.

关 键 词：沉香精油 纤维素酶 提取 

分 类 号：R284.2[医药卫生—中药学]